AI Article Synopsis
- The text outlines protocols for studying the Golgi structure using thin section transmission electron microscopy (TEM) and DAB cytochemical staining.
- It highlights methods for chemical fixation and flat embedding to ensure accurate imaging of the Golgi apparatus.
- The importance of classifying Golgi membranes through software like Microscopy Image Browser for better understanding Golgi morphology is also emphasized.
Article Abstract
Here, we describe protocols for chemical fixation and flat embedding to study the Golgi structure by thin section transmission electron microscopy (TEM) and for 3,3'-diaminobenzidine (DAB) cytochemical staining and pre-embedding immunolabelling to localize specific Golgi proteins. Furthermore, we demonstrate how the Golgi morphology can be elucidated by classifying the Golgi membranes using Microscopy Image Browser-a software that provides anonymization, modelling, and annotation.
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| http://dx.doi.org/10.1007/978-1-0716-2639-9_11 | DOI Listing |
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