AI Article Synopsis
- Scientists have used something called the HMQC pulse sequence to study big protein groups, which are special proteins made up of many smaller pieces.
- This method helps them see different types of signals from these proteins more clearly.
- They even created a faster version of the method that makes it easier to see important details in proteins that weigh between 100,000 to 1,000,000 times heavier than a hydrogen atom!
Article Abstract
The HMQC pulse sequence and variants thereof have been exploited in studies of high molecular weight protein complexes, taking advantage of the fact that fast and slow relaxing magnetization components are sequestered along two distinct magnetization transfer pathways. Despite the simplicity of the HMQC scheme an even shorter version can be designed, based on elimination of the terminal refocusing period, as a further means of increasing signal. Here we present such an experiment, and show that significant sensitivity gains, in some cases by factors of two or more, are realized in studies of proteins varying in molecular masses from 100 kDa to 1 MDa.
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| http://dx.doi.org/10.1016/j.jmr.2022.107326 | DOI Listing |
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