The food enzyme phytepsin (EC 3.4.23.40) is extracted from the pistils of cardoon ( L.) by QUALIFICA/oriGIn PORTUGAL. It is intended to be used in milk processing for cheese production. As no concerns arose from the source of the food enzyme, from its manufacture, and based on the history of safe use and consumption, the Panel considered that toxicological data and the estimation of dietary exposure were not required. The Panel considered that allergic reactions to this phytepsin cannot be excluded in individuals allergic to this plant. However, the likelihood of allergic reactions to the phytepsin from L. is expected not to exceed the likelihood of allergic reactions to cardoon. As the prevalence of allergic reactions to cardoon is low, also the likelihood of such reaction to occur to the food enzyme is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use.
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http://dx.doi.org/10.2903/j.efsa.2022.7678 | DOI Listing |
Anal Chim Acta
February 2025
Nanobiophotonics Department, Leibniz Institute of Photonic Technology (Leibniz-IPHT), Albert-Einstein-Strasse 9, 07745, Jena, Germany. Electronic address:
In recent years, nanozyme-based analytics have become popular. Among these, laccase nanozyme-based colorimetric sensors have emerged as simple and rapid colorimetric detection methods for various analytes, effectively addressing natural enzymes' stability and high-cost limitations. Laccase nanozymes are nanomaterials that exhibit inherent laccase enzyme-like activity.
View Article and Find Full Text PDFJ Ethnopharmacol
January 2025
Jiangxi Key Laboratory of Natural Product and Functional Food, College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China. Electronic address:
Ethnopharmacological Relevance: As an important component of the cell wall of Gram-negative bacteria, lipopolysaccharide (LPS) is an important inducer of inflammation in humans. Smilax china L. is known for its diverse bioactive functions, particularly its anti-inflammatory effects.
View Article and Find Full Text PDFJ Biotechnol
January 2025
Analysis and Testing Center, Nanjing Normal University, Nanjing, 210023, China. Electronic address:
Chondroitin sulfate (CS) is a structurally complex anionic polysaccharide widely used in medical, cosmetic and food applications. Enzymatic catalysis is an important strategy for synthesizing CS with uniform chain lengths and well-defined structures. However, the industrial application of glycosyltransferases is hindered by limitations such as low expression yields, poor stability, and challenges in reuse.
View Article and Find Full Text PDFBioresour Technol
January 2025
Water Research Centre and Department of Civil and Environmental Engineering, University of Auckland, Auckland 1142, New Zealand. Electronic address:
Dynamic oxygen fluctuations in activated sludge were investigated to enhance valuable biochemical production during wastewater treatment. Batch experiments compared constant aeration with rapid cycling between oxygen-rich and oxygen-poor states. Fluctuating oxygen concentrations (0-2 mg/L) significantly increased production of valuable biochemicals compared to constant oxygen concentration (2 mg/L).
View Article and Find Full Text PDFEcotoxicol Environ Saf
January 2025
College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China; Key Laboratory of the Provincial Education Department of Heilongjiang for Common Animal Disease Prevention and Treatment, Harbin 150030, China. Electronic address:
Bisphenol A (BPA) is a common endocrine disruptor chemical that is widely used in the production of food plastic packaging, and it has been shown to potentially harm the reproductive system. However, the specific mechanism by which BPA induces apoptosis of Leydig cells (LCs) and inhibits testosterone synthesis in these cells is unclear. In the present study, TM3 cells were used as an experimental model in combination with a reactive oxygen species (ROS) scavenger (N-acetylcysteine), Caspase-3 inhibitor (Ac-DEVD-CHO), autophagy activator (Torin2), and autophagy inhibitor (Chloroquine) to investigate the potential mechanisms by which BPA causes TM3 cell damage in vitro.
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