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Pharmazentrum Frankfurt/ZAFES, Institute of Pharmacology and Toxicology, Hospital of the Goethe University, Frankfurt am Main, Germany.
Published: December 2022
AI Article Synopsis
Article Abstract
Background: Cytotoxic T lymphocytes take on a leading role in many immune-related diseases. They function as key effector immune cells fighting cancer cells, but they are also considerably involved in autoimmune diseases. Common to both situations, CD8 T cells need to adapt their metabolism and effector function to the harsh and nutrient-deprived conditions of the disease-associated microenvironment.
Methods: We used an in vitro starvation as well as rapamycin treatment protocol mimicking nutrient deprivation to generate CD8 versus CD8 T cells and performed FACS-Sorting followed by transcriptomic profiling of the cytotoxic T cell subsets. Prominent markers identified in the CD8 versus the CD8 T cells were then used to investigate the presence of these cell subsets in immune-related human diseases. Employing cancer tissue microarrays and PhenOptics multispectral imaging as well as flow cytometry, we studied these CD8 T cell subsets in cancer and relapsing-remitting multiple sclerosis patients.
Results: Starvation induced a decreased expression of CD8, yielding a CD8 T cell subpopulation with an altered transcriptomic signature and reduced effector function. CD8 T cell showed enhanced ST2L and IL6ST (CD130) expression compared to CD8 T cells which expressed elevated KLRD1 (CD94) and granzyme B levels within the tumour microenvironment (TME). Spatial analysis revealed the presence of CD8 T cells in close proximity to tumour cells, while the CD8 T cells resided at the tumour boundaries. Importantly, the number of tumour-infiltrating CD8 T lymphocytes correlated with a poor prognosis as well as with enhanced cancer progression in human mammary carcinoma. We also found a reduced frequency of CD8 T lymphocytes in a cohort of relapse (disease active) multiple sclerosis patients compared to healthy subjects during immune cell starvation in vitro.
Conclusions: In summary, our data show that functionally distinct cytotoxic T lymphocytes can be identified based on their expression of CD8. Indicating a more general role in CD8 T cell immunity, these cells may play opposing roles in the TME, and also in the pathophysiology of autoimmune diseases such as multiple sclerosis.
