AI Article Synopsis
- Vestibular hair cells (V-HCs) in the inner ear are crucial for balance, but effective methods to differentiate pluripotent stem cells into V-HCs are still being developed.
- Recent research utilized vestibular cell-derived conditioned medium (V-CM) to enhance the differentiation of embryonic stem cells into V-HC-like cells when cultured in three-dimensional (3D) environments.
- The study found that 3D cultures showed significantly higher expression of V-HC markers compared to two-dimensional cultures, suggesting that this method, combined with V-CM, is promising for producing V-HCs.
Article Abstract
Vestibular hair cells (V-HCs) residing in the inner ear have important roles related to balance. Although differentiation of pluripotent stem cells into HCs has been shown, an effective method has yet to be established. We previously reported that use of vestibular cell-derived conditioned medium (V-CM) was helpful to induce embryonic stem (ES) cells to differentiate into V-HC-like cells in two-dimensional (2D) cultures of ES-derived embryoid bodies (EBs). In the present report, V-CM was used with three-dimensional (3D) cultures of EBs, which resulted in augmented expression of V-HC-related markers (Math1, Myosin6, Brn3c, Dnah5), but not of the cochlear HC-related marker Lmod3. Gene expression analyses of both 2D and 3D EBs cultured for two weeks revealed a greater level of augmented induction of HC-related markers in the 3D-cultured EBs. These results indicate that a 3D culture in combination with use of V-CM is an effective method for producing V-HCs.
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| http://dx.doi.org/10.1016/j.jbiosc.2022.11.005 | DOI Listing |
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