ROS and RNS Alterations in the Digestive Fluid of × Trap at Different Developmental Stages.

The carnivorous pitcher plant, × (Hort. ex Fleming = Blanco × Blanco), produces passive traps containing digestive fluid. Although reactive oxygen species (ROS) in the fluid were detected in some pitcher plants, the participation of reactive nitrogen species (RNS) in the digestion process has not yet been examined. The aim of this work was to investigate the production of superoxide anion (O), nitric oxide (NO) and peroxynitrite (ONOO) levels in the digestive fluid of traps throughout organ development. We revealed the ROS and RNS occurrence in the digestive fluid, linked to the ROS-scavenging capacity and total phenolics content. In digestive fluid from the fed traps, NO emission was higher than in the fluid from the developed unfed pitcher. The concentration of nitrite (NO) decreased in the fluid from the fed traps in comparison to the unfed ones, pointing at NO as the key source of NO. The enhanced emission of NO was associated with lowered content of ONOO in the fluid, probably due to lower production of O. At the same time, despite a decline in total phenolics, the maximum ROS scavenging capacity was detected. In addition, ROS and RNS were noted even in closed traps, suggesting their involvement not only in digestion per se but also their action as signaling agents in trap ontogeny.