Cytotoxicity of Extracts from Leaves on Yeast.

L. is a plant used in traditional medicine harboring pharmacological properties with anti-inflammatory, antinociceptive, hypoglycemiant and anesthetic activities. This study assessed the potential cytotoxic, genotoxic and mutagenic effects of ethanolic extract of on strains. FF18733 (wild type) and CD138 () strains were exposed to fractioned ethanolic extracts of in different concentrations. Three experimental assays were performed: cellular inactivation, mutagenesis (canavanine resistance system) and loss of mitochondrial function (petites colonies). The chemical analyses revealed a rich extract with phenolic compounds such as protocatechuic acid, cinnamic and catechin epicatechin. A decreased cell viability in wild-type and strains was demonstrated. All fractions of the extract exerted a mutagenic effect on the strain. Only ethyl acetate and n-butanol fractions increased the rate of petites colonies in the strain, but not in the wild-type strain. The results indicate that fractions of mid-polarity of the ethanolic extract, at the studied concentrations, can induce mutagenicity mediated by oxidative lesions in the mitochondrial and genomic genomes of the ogg1-deficient strain. These findings indicate that the lesions caused by the fractions of ethanolic extract can be mediated by reactive oxygen species and can reach multiple molecular targets to exert their toxicity.