Comparative Transcriptome Analysis of CCCH Family in Roles of Flower Opening and Abiotic Stress in .

CCCH is a zinc finger family with a typical CCCH-type motif which performs a variety of roles in plant growth and development and responses to environmental stressors. However, the information about this family has not been reported for . In this study, a total of 66 predicted genes were identified from the genome, the majority of which had multiple CCCH motifs. The 66 were found to be unevenly distributed on 21 chromosomes and were clustered into nine groups based on their phylogenetic analysis. In each group, the gene structure and domain makeup were comparatively conserved. The expression profiles of the OfCCCH genes were examined in various tissues, the flower-opening processes, and under various abiotic stresses using transcriptome sequencing and qRT-PCR (quantitative real-time PCR). The results demonstrated the widespread expression of in various tissues, the differential expression of 22 during flower-opening stages, and the identification of 4, 5, and 13 s after ABA, salt, and drought stress treatment, respectively. Furthermore, characterization of the representative OfCCCHs (OfCCCH8, 23, 27, and 36) revealed that they were all localized in the nucleus and that the majority of them had transcriptional activation in the yeast system. Our research offers the first thorough examination of the family and lays the groundwork for future investigations regarding the functions of CCCH genes in .