Rapid Detection of in Wine by Polychromatic Flow Cytometry.

is found in several fermented matrices and produces relevant alterations to the wine quality. The methods usually used to identify contamination are based on conventional microbiological techniques that require long procedures (15 days), causing the yeast to spread in the meantime. Recently, a flow cytometry kit for the rapid detection (1-2 h) of in wine has been developed. The feasibility of the method was assessed in a synthetic medium as well as in wine samples by detecting in the presence of other yeast species ( and spp.) and at the concentrations that produce natural contaminations (up to 10 cells/mL), as well as at lower concentrations (10-10 cells/mL). Wine samples naturally contaminated by or inoculated with four different strains of species together with and spp., were analyzed by flow cytometry. Plate counts were carried out in parallel to flow cytometry. We provide evidence that flow cytometry allows the rapid detection of in simple and complex mixtures. Therefore, this technique has great potential for the detection of and could allow preventive actions to reduce wine spoilage.