Oleic Acid Facilitates Cd Excretion by Increasing the Abundance of in Cd-Exposed Mice.

As a global pollutant, cadmium (Cd) can easily enter the body through food chains, threatening human health. Most Cd is initially absorbed in the gut, with the gut microbiota playing a pivotal role in reducing Cd absorption and accumulation. This study assessed the effects of three fatty acids on Cd accumulation and toxicity in Cd-exposed mice. The results showed that oleic acid (OA) was the most effective in facilitating Cd excretion in mice among these fatty acids. The use of OA led to reduced Cd accumulation in the organs and increased Cd content in the feces. The metagenomic analysis of the gut microbiota showed that the genus was the most significantly restored by OA in Cd-exposed mice. , as the type species for the genus , also exhibited strong Cd tolerance after treatment with OA. Furthermore, the electron microscopy analysis showed that most of the Cd was adsorbed on the surface of , where the extracellular polymeric substances (EPSs) secreted by play a key role, displaying a strong capacity for Cd adsorption. The peak at 2355 cm and the total sulfhydryl group content of EPSs showed significant increases following co-treatment with Cd and OA. The results demonstrated the potential roles that gut may play in OA-mediated Cd excretion in mice.