Cisplatin (CP), which is a conventional cancer chemotherapeutic drug, induces apoptosis by modulating a diverse array of gene regulatory mechanisms. However, cisplatin-mediated changes in the mA methylome are unknown. We employed an mA miCLIP-seq approach to investigate the effect of mA methylation marks under cisplatin-mediated apoptotic conditions on HeLa cells. Our high-resolution approach revealed numerous mA marks on 972 target mRNAs with an enrichment on 132 apoptotic mRNAs. We tracked the fate of differentially methylated candidate mRNAs under knockdown and cisplatin treatment conditions. Polysome profile analyses revealed perturbations in the translational efficiency of and transcripts. Congruently, amounts were dependent on . Additionally, cisplatin-mediated apoptosis was sensitized by knockdown. These results suggest that apoptotic pathways are modulated by mA methylation events and that the axis modulates cisplatin-mediated apoptosis in HeLa cells.
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| http://dx.doi.org/10.3390/cells11233905 | DOI Listing |
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