AI Article Synopsis
- Medium-chain-length fatty alcohols are valuable in various industries, including surfactants, lubricants, and cosmetics, with their acetate esters commonly used in flavors and fragrances.
- Researchers engineered strains of Pseudomonas putida to enhance its ability to produce 1-hexanol and hexyl acetate by removing genes responsible for degrading fatty alcohols.
- The optimal engineered strain produced significant amounts of 1-hexanol and hexyl acetate, suggesting P. putida can be developed as a microbial factory for sustainable production of medium-chain-length fatty alcohols and their products.
Article Abstract
Medium-chain-length fatty alcohols have broad applications in the surfactant, lubricant, and cosmetic industries. Their acetate esters are widely used as flavoring and fragrance substances. Pseudomonas putida KT2440 is a promising chassis for fatty alcohol and ester production at the industrial scale due to its robustness, versatility, and high oxidative capacity. However, P. putida has also numerous native alcohol dehydrogenases, which lead to the degradation of these alcohols and thereby hinder its use as an effective biocatalyst. Therefore, to harness its capacity as a producer, we constructed two engineered strains (WTΔpedFΔadhP, GN346ΔadhP) incapable of growing on mcl-fatty alcohols by deleting either a cytochrome c oxidase PedF and a short-chain alcohol dehydrogenase AdhP in P. putida or AdhP in P. putida GN346. Carboxylic acid reductase, phosphopantetheinyl transferase, and alcohol acetyltransferase were expressed in the engineered P. putida strains to produce hexyl acetate. Overexpression of transporters further increased 1-hexanol and hexyl acetate production. The optimal strain G23E-MPAscTP produced 93.8 mg/L 1-hexanol and 160.5 mg/L hexyl acetate, with a yield of 63.1%. The engineered strain is applicable for C-C fatty alcohols and their acetate ester production. This study lays a foundation for P. putida being used as a microbial cell factory for sustainable synthesis of a broad range of products based on medium-chain-length fatty alcohols.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ymben.2022.11.006 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Long-chain fatty acids as sole carbon source in polyhydroxyalkanoates production by Cupriavidus necator H16.
Bioresour Technol
February 2025
University of Oviedo, Department of Chemical Engineering and Environmental Technology. Julián Clavería 8, Faculty of Chemistry, Oviedo, Spain. Electronic address:
Polyhydroxyalkanoates (PHA) are promising eco-friendly alternatives to petrochemical plastics. This study investigated the impact of the main fatty acids present in waste and fresh oils -palmitic, stearic, oleic, and linoleic acid-on PHA production using Cupriavidus necator H16, focusing on production yield, polymer composition, thermal properties, and microbial viability. Experiments were conducted with low (5 g/L) and high (15 g/L) carbon content for 168 h.
View Article and Find Full Text PDFPossibilities and prospects of bioplastics production from agri-waste using bacterial communities: Finding a silver-lining in waste management.
Curr Res Microb Sci
September 2024
Laboratory of Applied Stress Biology, Department of Botany, University of Gour Banga, Malda - 732 103, West Bengal, India.
To meet the need of the growing global population, the modern agriculture faces tremendous challenges to produce more food as well as fiber, timber, biofuels, etc.; hence generates more waste. This continuous growth of agricultural waste (agri-waste) and its management strategies have drawn the attention worldwide because of its severe environmental impacts including air, soil and water pollution.
View Article and Find Full Text PDFCross-family transfer of the Arabidopsis cell-surface immune receptor LORE to tomato confers sensing of 3-hydroxylated fatty acids and enhanced disease resistance.
Mol Plant Pathol
September 2024
TUM School of Life Sciences, Technical University of Munich, Freising-Weihenstephan, Germany.
Plant pathogens pose a high risk of yield losses and threaten food security. Technological and scientific advances have improved our understanding of the molecular processes underlying host-pathogen interactions, which paves the way for new strategies in crop disease management beyond the limits of conventional breeding. Cross-family transfer of immune receptor genes is one such strategy that takes advantage of common plant immune signalling pathways to improve disease resistance in crops.
View Article and Find Full Text PDFS211 as a microbial cell factory for direct bioconversion of waste cooking oil into medium-chain-length polyhydroxyalkanoates.
3 Biotech
September 2024
APVA-LR16ES20, National School of Engineers of Sfax (ENIS), University of Sfax, Sfax, Tunisia.
The present study examines the use of waste cooking oil (WCO) as a substrate for medium-chain-length polyhydroxyalkanoates (mcl-PHA) production by S211. The genome analysis revealed that the S211 strain has a mcl-PHA cluster () encoding two class II PHA synthases (PhaC1 and PhaC2) separated by a PHA depolymerase (PhaZ), a transcriptional activator (PhaD) and two phasin-like proteins (PhaFI). Genomic annotation also identified a gene encoding family I.
View Article and Find Full Text PDFCorroborative studies on chain packing characteristics of biological medium-chain-length poly-3-hydroxyalkanoates with different monomeric composition.
Int J Biol Macromol
June 2024
Institute of Biological Sciences, Faculty of Science, Universiti Malaya, 50603 Kuala Lumpur, Malaysia.
Medium-chain-length poly-3-hydroxyalkanoates (mcl-PHAs) with varied monomeric compositions were biosynthesized by producer bacteria fed with different fatty acids as carbon source. Octanoic-, lauric-, stearic-, and oleic acids were used to produce four types of mcl-PHAs viz. PHA-OC, PHA-LA, PHA-ST, and PHA-OL, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!