AI Article Synopsis
- A comprehensive multiomic cell atlas of human lung development was created using advanced techniques like single-cell RNA and ATAC sequencing, spatial transcriptomics, and imaging, covering a developmental period from 5 to 22 post-conception weeks.
- The study identified previously unknown cell types across various lung compartments, including specific progenitor cells and a neuroendocrine cell subtype linked to small cell lung cancer.
- The data is accessible online for research purposes, and the atlas enabled predictions about cell interactions and transcription factors, which were tested using organoid models.
Article Abstract
We present a multiomic cell atlas of human lung development that combines single-cell RNA and ATAC sequencing, high-throughput spatial transcriptomics, and single-cell imaging. Coupling single-cell methods with spatial analysis has allowed a comprehensive cellular survey of the epithelial, mesenchymal, endothelial, and erythrocyte/leukocyte compartments from 5-22 post-conception weeks. We identify previously uncharacterized cell states in all compartments. These include developmental-specific secretory progenitors and a subtype of neuroendocrine cell related to human small cell lung cancer. Our datasets are available through our web interface (https://lungcellatlas.org). To illustrate its general utility, we use our cell atlas to generate predictions about cell-cell signaling and transcription factor hierarchies which we rigorously test using organoid models.
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| http://dx.doi.org/10.1016/j.cell.2022.11.005 | DOI Listing |
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