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Identification and characterization of , a major gene controlling leaf yellowing in cucumber. | LitMetric

Mutants are crucial to extending our understanding of genes and their functions in higher plants. In this study a spontaneous cucumber mutant, , showed yellow color leaves, had significant decreases in related physiological indexes of photosynthesis characteristics, and had more abnormal chloroplasts and thylakoids. Inheritance analysis indicated that the yellow color of the leaf was controlled by a recessive nuclear locus, . A candidate gene, , encoding a chloroplast signal recognition particle 43 protein, was identified through map-based cloning and whole-genome sequence analysis. Alignment of the gene homologs between both parental lines revealed a 7-kb deletion mutation including the promoter region and the coding sequence in the mutant. In order to determine if the gene was involved in the formation of leaf color, the CRISPR/Cas9-mediate system was used to modify in the 9930 background; two independent transgenic lines, and , were generated, and they showed yellow leaves with abnormal chloroplasts and thylakoids. Transcriptomic analysis revealed that differentially expressed genes associated with the photosynthesis-related pathway were highly enriched between and wild type, most of which were significantly downregulated in line . Furthermore, yeast two-hybrid and biomolecular fluorescence complementation assays were used to confirm that CsSRP43 directly interacted with LHCP and cpSRP54 proteins. A model was established to explain the molecular mechanisms by which CsSRP43 participates in the leaf color and photosynthesis pathway, and it provides a valuable basis for understanding the molecular and genetic mechanisms of leaf color in cucumber.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9719040PMC
http://dx.doi.org/10.1093/hr/uhac212DOI Listing

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