Molecular methods to study protein trafficking between organs.

Proteomics

Department of Molecular Medicine, Scripps Research, La Jolla, California, USA.

Published: March 2023

Interorgan communication networks are key regulators of organismal homeostasis, and their dysregulation is associated with a variety of pathologies. While mass spectrometry proteomics identifies circulating proteins and can correlate their abundance with disease phenotypes, the tissues of origin and destinations of these secreted proteins remain largely unknown. In vitro approaches to study protein secretion are valuable, however, they may not mimic the complexity of in vivo environments. More recently, the development of engineered promiscuous BirA* biotin ligase derivatives has enabled tissue-specific tagging of cellular secreted proteomes in vivo. The use of biotin as a molecular tag provides information on the tissue of origin and destination, and enables the enrichment of low-abundance hormone proteins. Therefore, promiscuous protein biotinylation is a valuable tool to study protein secretion in vivo.

Download full-text PDF

Source
http://dx.doi.org/10.1002/pmic.202100331DOI Listing

Publication Analysis

Top Keywords

study protein
12
protein secretion
8
molecular methods
4
methods study
4
protein
4
protein trafficking
4
trafficking organs
4
organs interorgan
4
interorgan communication
4
communication networks
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!