Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a global coronavirus disease 2019 (COVID-19) pandemic that has affected the lives of billions of individuals. However, the host-virus interactions still need further investigation to reveal the underling mechanism of SARS-CoV-2 pathogenesis. Here, transcriptomics analysis of SARS-CoV-2 infection highlighted possible correlation between host-associated signaling pathway and virus. In detail, cAMP-protein kinase (PKA) pathway has an essential role in SARS-CoV-2 infection, followed by the interaction between cyclic AMP response element binding protein (CREB) and CREB-binding protein (CBP) could be induced and leading to the enhancement of CREB/CBP transcriptional activity. The replication of Delta and Omicron BA.5 were inhibited by about 49.4% and 44.7% after knockdown of CREB and CBP with small interfering RNAs, respectively. Furthermore, a small organic molecule naphthol AS-E (nAS-E), which targets on the interaction between CREB and CBP, potently inhibited SARS-CoV-2 wild-type (WT) infection with comparable the half-maximal effective concentration (EC ) 1.04 μM to Remdesivir 0.57 μM. Compared with WT virus, EC in Calu-3 cells against Delta, Omicron BA.2, and Omicron BA.5 were, on average, 1.5-fold, 1.1-fold, and 1.5-fold higher, respectively, nAS-E had a satisfied antiviral effect against Omicron variants. Taken together, our study demonstrated the importance of CREB/CBP induced by cAMP-PKA pathway during SARS-CoV-2 infection, and further provided a novel CREB/CBP interaction therapeutic drug targets for COVID-19.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9877775 | PMC |
| http://dx.doi.org/10.1002/jmv.28383 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Changes in Grocery Shopping Behavior among Low-Income Households during the COVID-19 Pandemic.
Public Health Nutr
January 2025
Department of Nutrition and Food Studies, Steinhardt School of Culture, Education, and Human Development, New York University, 411 Lafayette St, 5th floor, New York, NY 10003.
Objective: The Supplemental Nutrition Assistance Program (SNAP) Online Purchasing Pilot (OPP) authorized the use of SNAP benefits online in Maryland in May 2020. We assessed shopping behavior and intentions associated with uptake and intended future use of online grocery shopping during and after COVID-19 among SNAP-eligible households.
Design: In this mixed-methods study, participants completed a survey on online grocery shopping, and a purposefully sampled subset participated in focus groups or in-depth interviews between November 2020 and March 2021.
Anatomy education in transition: examining medical student experiences during the distance education.
Folia Morphol (Warsz)
January 2025
Tekirdağ Namık Kemal University School of MedicineTekirdağ, Türkiye.
Background: Distance education emerged as a potential solution to enhance access, standardize content, and facilitate updates. However, student perceptions varied widely. The COVID-19 pandemic prompted a rapid shift towards distance education in anatomy, presenting challenges and opportunities for medical students globally.
View Article and Find Full Text PDFPerspectives on postbaccalaureate PEERS: Advocacy and empowering communities.
Biochem Mol Biol Educ
January 2025
Heritage University, Toppenish, Washington, USA.
The impact of Covid-19 pandemic has dramatically shifted the education landscape between recent college and university graduates and pathways to graduate degrees. In my perspective article, I wish to share the challenges, reflections, and a call-to-action framework in ways we can support and advocate for postbaccalaureate persons excluded because of their ethnicity of race, or from a structurally marginalized community or PEERS through the lens of mindfulness, humility, reflection, and deep listening. Through cross-institutional community network support, culturally responsive mentoring of postbaccalaureate PEERS is one of the key dimensions in empowering communities toward health, environmental, and social justice.
View Article and Find Full Text PDFCan there be calm during a cytokine storm? Immune checkpoint pathways affecting the severity of COVID-19 disease.
Front Microbiol
December 2024
Department of Medical Microbiology and Immunology, Medical School, University of Pecs, Pecs, Hungary.
Introduction: The COVID-19 pandemic has become a global health crisis, eliciting varying severity in infected individuals. This study aimed to explore the immune profiles between moderate and severe COVID-19 patients experiencing a cytokine storm and their association with mortality. This study highlights the role of PD-1/PD-L1 and the TIGIT/CD226/CD155/CD112 pathways in COVID-19 patients.
View Article and Find Full Text PDFIn-Hospital Mortality by Race and Ethnicity Among Hospitalized COVID-19 Patients Using Data From the US National COVID Cohort Collaborative.
Am J Med Open
December 2024
Department of Biomedical Informatics, University of Arkansas for Medical Sciences, Little Rock, AR.
Background: Studies examining racial and ethnic disparities in-hospital mortality for patients hospitalized with COVID-19 had mixed results. Findings from patients within academic medical centers (AMCs) are lacking, but important given the role of AMCs in improving health equity.
Objective: The purpose of this study is to assess whether minority patients hospitalized with COVID-19 in National COVID Cohort Collaborative (N3C) institutions, which consist predominantly of AMCs, have higher mortality rates relative to White patients.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!