AI Article Synopsis
- Proteins serve as vital biocatalysts, with their functions directly linked to their structures, making protein engineering essential for enhancing enzyme stability and efficiency.
- Traditional metabolic engineering has aimed at boosting metabolic pathways using gene expression and enzyme concentration, but limitations in natural enzymes have restricted their practical use.
- Recent advancements in protein engineering across synthetic biology, chemoenzymatic synthesis, and plant metabolic engineering present new opportunities for creating innovative enzymes with improved functions to produce valuable natural products.
Article Abstract
Proteins are the most frequently used biocatalysts, and their structures determine their functions. Modifying the functions of proteins on the basis of their structures lies at the heart of protein engineering, opening a new horizon for metabolic engineering by efficiently generating stable enzymes. Many attempts at classical metabolic engineering have focused on improving specific metabolic fluxes and producing more valuable natural products by increasing gene expression levels and enzyme concentrations. However, most naturally occurring enzymes show limitations, and such limitations have hindered practical applications. Here we review recent advances in protein engineering in synthetic biology, chemoenzymatic synthesis, and plant metabolic engineering and describe opportunities for designing and constructing novel enzymes or proteins with desirable properties to obtain more active natural products.
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| http://dx.doi.org/10.1016/j.tplants.2022.11.004 | DOI Listing |
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