AI Article Synopsis
- PIWI-interacting RNAs (piRNAs) are small RNAs that help silence transposable elements and are connected to specific Argonaute proteins.
- In male mouse germ cells, piRNAs start to develop around E13.5, but research on in vitro models of germ cells shows that key piRNA factors are not expressed at Day 6 of differentiation.
- The findings indicate that Day 6 mPGCLCs do not yet mimic the active piRNA pathway seen in more developed gonocytes, challenging assumptions about their development.
Article Abstract
PIWI-interacting RNAs (piRNAs) are small RNAs bound by PIWI-clade Argonaute proteins that function to silence transposable elements (TEs). Following mouse primordial germ cell (mPGC) specification around E6.25, fetal piRNAs emerge in male gonocytes from E13.5 onward. The in vitro differentiation of mPGC-like cells (mPGCLCs) has raised the possibility of studying the fetal piRNA pathway in greater depth. However, using single-cell RNA-seq and RT-qPCR along mPGCLC differentiation, we find that piRNA pathway factors are not fully expressed in Day 6 mPGCLCs. Moreover, we do not detect piRNAs across a panel of Day 6 mPGCLC lines using small RNA-seq. Our combined efforts highlight that in vitro differentiated Day 6 mPGCLCs do not yet resemble E13.5 or later mouse gonocytes where the piRNA pathway is active. This Matters Arising paper is in response to von Meyenn et al. (2016), published in Developmental Cell. See also the correction by von Meyenn et al. published in this issue.
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| http://dx.doi.org/10.1016/j.devcel.2022.11.004 | DOI Listing |
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