AI Article Synopsis
- VLP technology is gaining popularity in science, with this study focusing on reporter-tagged VLPs of the CHIKV, an RNA virus, and their applications in research.
- Various methods like Western blot and live-cell imaging confirmed successful expression and characteristics of these CHIK-VLPs, which are around 67nm in diameter.
- The findings suggest that these reporter-tagged VLPs can be used effectively to detect CHIKV in serum samples and also evaluate the effectiveness of the Suramin drug as an inhibitor of the virus's entry, paving the way for advancements in antiviral testing.
Article Abstract
The ever-evolving and versatile VLP technology is becoming an increasingly popular area of science. This study presents surface decorated reporter-tagged VLPs of CHIKV, an enveloped RNA virus of the genus alphavirus and its applications. Western blot, IFA and live-cell imaging confirm the expression of reporter-tagged CHIK-VLPs from transfected HEK293Ts. CryoEM micrographs reveal particle diameter as ∼67nm and 56-70 nm, respectively, for NLuc CHIK-VLPs and mCherry CHIK-VLPs. Our study demonstrates that by exploiting NLuc CHIK-VLPs as a detector probe, robust ratiometric luminescence signal in CHIKV-positive sera compared to healthy controls can be achieved swiftly. Moreover, the potential activity of the Suramin drug as a CHIKV entry inhibitor has been validated through the reporter-tagged CHIK-VLPs. The results reported in this study open new avenues in the eVLPs domain and offer potential for large-scale screening of clinical samples and antiviral agents targeting entry of CHIKV and other alphaviruses.
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| http://dx.doi.org/10.1016/j.virol.2022.11.012 | DOI Listing |
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