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[Proteomic study of Jingfang Mixture on urticaria based on label-free quantitative proteomics technology]. | LitMetric

[Proteomic study of Jingfang Mixture on urticaria based on label-free quantitative proteomics technology].

Zhongguo Zhong Yao Za Zhi

State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine Linyi 276006, China.

Published: October 2022

AI Article Synopsis

  • This study looked at how a mixture called Jingfang affects mice with a skin condition called urticaria (like hives) and how it works.
  • The researchers divided 27 male mice into three groups: one normal, one that got a skin allergy, and one that got the Jingfang mixture to see if it would help.
  • They found 76 proteins changed in the skin of the mice that got the Jingfang mixture, which could help understand how it helps with energy and the immune system in the skin.

Article Abstract

This study aims to explore the effect of Jingfang Mixture on the protein expression of urticaria in mice and explain the mechanism of Jingfang Mixture in the treatment of urticaria. Twenty-seven male Kunming mice were randomly divided into a normal group, a model group and a Jingfang Mixture group according to body weight. Except for the normal group, mice in the model group and the Jingfang Mixture group were injected with the mixture of ovalbumin and Al(OH)_3 gel for the first immunization, and the second immunization was performed on the 10 th day to induce the urticaria model. Mice in the Jingfang Mixture group started to be administered on the 6 th day after the initial immunization, and was administered continuously for 21 days. The normal group and the model group were replaced with the same amount of purified water. Twenty-four hours after the last administration, an appropriate amount of skin was taken, and label-free quantitative proteomics technology was used to detect the differences in protein expression in skin tissue. The signaling pathways involved in the differential proteins was further analyzed. The results of proteomics indicated that seventy-six proteins were involved in the intervention of Jingfang Mixture on mice with urticaria, and the differential proteins were mainly enriched in biological process(BP), molecular function(MF), and cellular component(CC). Kyoto Encyclopedia of Genes and Genomes(KEGG) analysis showed that the signaling pathways regulated by Jingfang Mixture mainly involved carbon metabolism, metabolic pathways, glucagon signaling pathway, glycolysis/gluconeogenesis, pentose phosphate pathway, hypoxia inducible factor-1(HIF-1) signaling pathway, purine metabolism, adherens junction, calcium signaling pathway, leukocyte transendothelial migration, and inflammatory mediator regulation of transient receptor potential(TRP) channels, which were involved in skin tissue energy metabolism and immune regulation. The findings of this study showed that the protective effect of Jingfang Mixture on mice with urticaria was closely related to the regulation of immune disorders, and the regulatory effect on immune system may be achieved through the regulation of energy metabolism by Jingfang Mixture.

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Source
http://dx.doi.org/10.19540/j.cnki.cjcmm.20220506.403DOI Listing

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