Human growth hormone supplement promotes human lymphohematopoietic cell reconstitution in immunodeficient mice.

To investigate the potential of human growth hormone (hGH) to improve human hematopoietic reconstitution in humanized mice. Immunodeficient mice were conditioned by total body irradiation and transplanted with human CD34 fetal liver cells. Peripheral blood, spleen and bone marrow were harvested, and levels of human lymphohematopoietic cells were determined by flow cytometry. Supplementation with hGH elevated human lymphohematopoietic chimerism by more than twofold. Treatment with hGH resulted in significantly increased reconstitution of human B cells and myeloid cells in lymphoid organs, enhanced human erythropoiesis in the bone morrow, and improved engraftment of human hematopoietic stem cells. hGH supplementation promotes human lymphohematopoietic reconstitution in humanized mice.