Regeneration is extremely important to pepper genetic development; however, the molecular mechanisms of how the callus reactivates cell proliferation and promotes cell reprogramming remain elusive in pepper. In the present study, (HNUCB81 and HNUCB226) and (HNUCC22 and HNUCC16) were analyzed to reveal callus initiation by regeneration, histology, and transcriptome. We successfully established an efficient regeneration system of two cultivars to monitor the callus induction of differential genotypes, and the regenerated plants were obtained. Compared to , there was a higher callus induction rate in . The phenotype of changed significantly and formed vascular tissue faster than . The KEGG enrichment analysis found that plant hormone transduction and starch and sucrose metabolism pathways were significantly enriched. In addition, we identified that the gene was significantly up-regulated in HNUCB81 and HNUCB226 than that in HNUCC22 and HNUCC16, which may be a potential function in callus formation. These results provided a promising strategy to improve the regeneration and transformation of pepper plants.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9714296 | PMC |
| http://dx.doi.org/10.3389/fpls.2022.1025497 | DOI Listing |
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