Introduction: Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam.
Material And Methods: The test performance was evaluated in a random selection of 160 previously molecularly characterized clinical isolates carrying the 110 bla, 1 bla, 12 bla, 4 bla, 3 bla and 42 bla genes. The proposed method relies on the detection of imipenem hydrolysis in an imipenem/relebactam antibiotic solution and subsequent visual interpretation by color change.
Results: All class A producing Enterobacterales (111/111) were detected using imipenem/relebactam as no visual appreciation of color change was perceived due to a nule hydrolysis of imipenem in the antibiotic solution. Overall, the assay showed 100% sensitivity (111/111) and specificity (69/69) for detecting class A KPC-producing Enterobacterales.
Discussion: The biochemical assay provides very reliable results for detecting KPC-producing Enterobacterales, with a turnaround time of less than 1 hour, minimum handling and no specialized equipment required.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.eimce.2021.03.010 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High-Copy IncP-2 Megaplasmid Carrying bla in Clinical Difficult-to-Treat Resistance Pseudomonas aeruginosa: Associated with High-Level Cefiderocol Resistance.
J Infect
January 2025
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China; Infection Control Department, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China. Electronic address:
Genomic insights into a multidrug-resistant Pandoraea apista clinical isolate carrying bla from China.
J Glob Antimicrob Resist
January 2025
Clinical Laboratory Department, Lishui People's Hospital, the Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, China. Electronic address:
Objectives: Pandoraea apista is notable for its multidrug resistance and is frequently identified in patients with cystic fibrosis or other chronic lung diseases, where it contributes to persistent lung infections. In this study, we describe a strain of P. apista harboring the bla, isolated from the bronchoalveolar lavage (BAL) fluid of an inpatient in China.
View Article and Find Full Text PDFInvestigating the Antibiotic Resistance Genes and Mobile Genetic Elements in Water Systems impacted with Anthropogenic Pollutants.
Environ Res
January 2025
ICMR- National Institute for Research in Environmental Health, Bhopal Bypass Road, Bhauri, Bhopal - 462030, Madhya Pradesh, India. Electronic address:
A wide range of pollutants, including heavy metals, endocrine-disrupting chemicals (EDCs), residual pesticides, and pharmaceuticals, are present in various water systems, many of which strongly drive the proliferation and dissemination of antimicrobial resistance genes (ARGs), heightening the antimicrobial resistance (AMR) crisis and creating a critical challenge for environmental and health management worldwide. This study addresses the impact of anthropogenic pollutants on AMR through an extensive analysis of ARGs and mobile genetic elements (MGEs) in urban wastewater, source water, and drinking water supplies in India. Results indicated that bla and bla were the dominant ARGs across all water systems, underscoring the prevalence and dominance of resistance against β-lactam antibiotics.
View Article and Find Full Text PDFAntibiotic Resistance Profile of Enterovirulent Isolates Harboring Broad-Spectrum Beta-Lactamase Genes in Cancer Patients at the Laquintinie Hospital in Douala, Littoral Region, Cameroon.
Int J Microbiol
January 2025
Department of Biochemistry, Faculty of Science, Université de Dschang, Dschang, Cameroon.
Cases of antibiotic-resistant () infections are becoming increasingly frequent and represent a major threat to our ability to treat cancer patients. The emergence of antimicrobial resistance threatens the treatment of infections. In this study, the antimicrobial profiles, virulent genes, and the frequency of extended-spectrum beta-lactamase (ESBL) gene carriage in fecal isolates from cancer patients at the Laquintinie Hospital in Douala (Cameroon) were determined.
View Article and Find Full Text PDFHouseflies (Musca domestica) as vectors of multidrug-resistant, ESBL-producing Escherichia coli in broiler poultry farms of North India: implications for antibiotic resistance transmission.
Environ Sci Pollut Res Int
January 2025
Gut Biology Laboratory, Room No. 117, Department of Zoology, University of Delhi, New Delhi, 110007, India.
The transmission of antibiotic resistance (AR) from farm animals to healthy human communities, beyond the food chain, is often facilitated by biological vectors, notably houseflies (Musca domestica). This study aimed to evaluate the role of M. domestica collected from commercial broiler chicken farms as a carrier of multidrug-resistant (MDR), extended-spectrum β-lactamase (ESBL)-producing Escherichia coli.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!