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Mechanism of autophagy induced by low concentrations of chlorantraniliprole in silk gland, Bombyx mori. | LitMetric

Mechanism of autophagy induced by low concentrations of chlorantraniliprole in silk gland, Bombyx mori.

Pestic Biochem Physiol

School of Basic Medicine and Biological Sciences, Soochow University, Suzhou, Jiangsu 215123, PR China; Sericulture Institute of Soochow University, Suzhou, Jiangsu 215123, PR China. Electronic address:

Published: November 2022

AI Article Synopsis

  • Chlorantraniliprole (CAP) is an agricultural pesticide that can negatively impact silkworm (Bombyx mori) cocoon formation, prompting the need for better toxicity assessment methods.
  • The study found that exposure to low concentrations of CAP decreases key ATPase activities in silkworms and significantly affects the regulation of AMPK-related and autophagy-related genes.
  • Overall, the findings highlight that CAP exposure causes autophagy in the silk glands of silkworms, impairing their physiological functions and aiding in evaluating the toxicity of environmental pesticide residues.

Article Abstract

Chlorantraniliprole (CAP) is widely used in the control of agricultural pests, and its residues can affect the formation of silkworm (Bombyx. mori) cocoon easily. To accurately evaluate the toxicity of CAP to silkworms and clarify the mechanism of its effect on silk gland function, we proposed a novel toxicity evaluation method based on the body weight changes after CAP exposure. We also analyzed the Ca-related ATPase activity, characterized energy metabolism and transcriptional changes about the autophagy key genes on the downstream signaling pathways. The results showed that after a low concentration of CAP exposed for 96 h, there were CAP residues in the silk glands of B. mori, the activities of Ca-ATPase and Ca-Mg-ATPase decreased significantly (P ≤ 0.01), and the activation of AMPK-related genes AMPK-α and AMPK-β were up-regulated by 6.39 ± 0.02-fold and 12.33 ± 1.06-fold, respectively, reaching a significant level (P ≤ 0.01)). In addition, the autophagy-related genes Atg1, Atg6, Atg5, Atg7, and Atg8 downstream AMPK were significantly up-regulated at 96 h (P ≤ 0.05). The results of immunohistochemistry and protein expression assay for autophagy marker Atg8 further confirmed the occurrence of autophagy. Overall, our results indicate that CAP exposure leads to autophagy in the silk gland of B. mori and affects their physiological functions, which provides guidance for the evaluation of toxicity of low concentration environmental CAP residues to insects.

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Source
http://dx.doi.org/10.1016/j.pestbp.2022.105223DOI Listing

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