E26 transformation-specific variant 2 (ETV2), as a member of Ets family of transcription factors, plays an important role in embryonic vasculogenesis, angiogenesis and hematopoiesis. Here, based on H9 cell line and PiggyBac technology, we generated a doxycycline-inducible ETV2 (Dox-ETV2-H9) embryonic stem cell (ESC) line. The Dox-ETV2-H9 cells maintain normal morphology, karyotype as well as high expression of pluripotent markers. In comparison with previous studies that employing costly ETV2 modRNA to improve differentiation from ESCs into endothelial cells (ECs), the tet-on system in Dox-ETV2-H9 cells could precisely manipulate ETV2 expression, which enables ECs differentiation process more efficient and controllable upon addition of doxycycline.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.scr.2022.102985 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
BCL6B-dependent suppression of ETV2 hampers endothelial cell differentiation.
Stem Cell Res Ther
July 2024
Department of Cardiology, Translational Research Center for Regenerative Medicine and 3D Printing Technologies, Guangdong Provincial Key Laboratory of Major Obstetric Diseases; Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, The Third Affiliated Hospital, Guangzhou Medical University, Guangzhou, 510150, China.
Background: B-cell CLL/lymphoma 6 member B (BCL6B) operates as a sequence-specific transcriptional repressor within the nucleus, playing crucial roles in various biological functions, including tumor suppression, immune response, stem cell self-renew, and vascular angiogenesis. However, whether BCL6B is involved in endothelial cell (EC) development has remained largely unknown. ETS variant transcription factor 2 (ETV2) is well known to facilitate EC differentiation.
View Article and Find Full Text PDFA streamlined method to generate endothelial cells from human pluripotent stem cells via transient doxycycline-inducible ETV2 activation.
Angiogenesis
November 2024
Department of Cardiac Surgery, Boston Children's Hospital, 300 Longwood Ave, Boston, MA, 02115, USA.
Article Synopsis
- The study introduces an efficient method for turning human induced pluripotent stem cells (iPSCs) into functional endothelial cells (iECs) using a doxycycline-inducible system to express the ETV2 transcription factor, achieving over 90% efficiency in just four days.
- This new method is designed to overcome challenges found in traditional transfection techniques, making it simpler and more consistent across different stem cell lines while allowing the iECs to maintain their ability to form blood vessels both in lab settings and in living organisms.
- The generated iECs show strong similarities to primary endothelial cells in terms of gene expression and protein profiles, validating their functionality and potential for use in research and
Generation of a doxycycline-inducible ETV2 expression cell line using PiggyBac transposase system.
Stem Cell Res
February 2023
Fuwai Hospital Chinese Academy of Medical Sciences, Shenzhen, China. Electronic address:
E26 transformation-specific variant 2 (ETV2), as a member of Ets family of transcription factors, plays an important role in embryonic vasculogenesis, angiogenesis and hematopoiesis. Here, based on H9 cell line and PiggyBac technology, we generated a doxycycline-inducible ETV2 (Dox-ETV2-H9) embryonic stem cell (ESC) line. The Dox-ETV2-H9 cells maintain normal morphology, karyotype as well as high expression of pluripotent markers.
View Article and Find Full Text PDFTransient ETV2 Expression Promotes the Generation of Mature Endothelial Cells from Human Pluripotent Stem Cells.
Biol Pharm Bull
April 2022
Laboratory of Biomedical Innovation, Graduate School of Pharmaceutical Sciences, Osaka University.
Differentiation protocols are used for induced pluripotent stem cells (iPSCs) in in vitro disease modeling and clinical applications. Transplantation of endothelial cells (ECs) is an important treatment strategy for ischemic diseases. For example, in vitro generated ECs can be used to provide the vascular plexus to regenerate organs such as the liver.
View Article and Find Full Text PDFEtv2 transcriptionally regulates Yes1 and promotes cell proliferation during embryogenesis.
Sci Rep
July 2019
Medicine Department and the Lillehei Heart Institute, University of Minnesota, Minneapolis, MN, 55455, USA.
Etv2, an Ets-transcription factor, governs the specification of the earliest hemato-endothelial progenitors during embryogenesis. While the transcriptional networks during hemato-endothelial development have been well described, the mechanistic details are incompletely defined. In the present study, we described a new role for Etv2 as a regulator of cellular proliferation via Yes1 in mesodermal lineages.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!