Can Capture CD86 Proteins of Macrophages in vitro.

Background: () is a thermally dimorphic fungus endemic to Southeast Asia that causes human systemic infection. Our earlier immunohistochemical studies revealed that the organisms were markedly labeled with the CD86 antigen in cutaneous lesions brought on by infection. However, the relationship between and the CD86 co-stimulatory molecule is still unknown.

Objective: To explore the association between CD86 Protein and organisms in vitro and discuss the potential mechanisms.

Methods: We created the CD86-EGFP fusion protein in THP-1 macrophages and co-cultured conidia with it. We used confocal fluorescence microscopy to view in vitro dynamics. The link between CD86 Protein and organisms in vitro was discovered using immunoelectron microscopy, indirect immunofluorescence test, and immunohistochemistry assay.

Results: cells received soluble CD86-EGFP from THP-1 macrophages detected by confocal fluorescent microscopy. Both the indirect immunofluorescence assay and the immunohistochemical assay showed that conidia were stained by the CD86 marker. Immunoelectron microscopy showed that characteristic colloidal gold particles were observed in organisms when co-cultured with THP-1 macrophages.

Conclusion: organisms have the ability to capture CD86 proteins from macrophages in vitro.