Evaluation of In Vivo Wound-Healing and Anti-Inflammatory Activities of Solvent Fractions of Fruits of L. (Papaveraceae).

Introduction: The solvent fractions of the fruits of L. (Papaveraceae) have not yet been explored scientifically for in vivo wound healing and anti-inflammatory activities. The objective of this study was, therefore, to evaluate in vivo wound healing and anti-inflammatory activities of the solvent fractions of the fruit of L. () in rats.

Method: The crude extract of was fractionated with -hexane, ethyl acetate, and distilled water. Wound healing activity was evaluated using excision and incision wound models while anti-inflammatory activity was evaluated using carrageenan-induced rat paw and cotton pellet-induced granuloma models. The fractions were evaluated at 5 and 10% ointments using moist-exposed burn ointment as the standard drug, and 100, 200, and 400 mg/kg test doses using aspirin, and dexamethasone as standard drugs for wound healing and anti-inflammatory activities, respectively. All treatment administrations were made orally for anti-inflammatory activity and applied topically for wound healing activity.

Result: The 10% w/w ethyl acetate fraction ointment showed a significant percentage of wound contraction, reduced period of epithelialization, increased amount of fibrosis, neovascularization, and collagen tissue formation ( < 0.01). The ethyl acetate fraction also showed a significant increase in tensile strength (55%; < 0.01) and (81.10%; < 0.01) at the tested doses of 5 and 10% w/w ointments, which was comparable to moist-exposed burn ointment. The ethyl acetate fraction also revealed a significant percent edema inhibition (61.41%; < 0.01), suppression of the exudate (38.09% < 0.01), and granuloma mass formations (53.47% < 0.01) at the tested dose of 400 mg/kg.

Conclusion: The results of this study showed that the Ethyl acetate fraction of fruit has significant wound healing and anti-inflammatory activities which support the traditional claims of the experimental plant.