AI Article Synopsis
- This study evaluates the accuracy and reliability of different testing methods, including test strips, ELISA kits, and UHPLC-MS/MS, for detecting mycotoxins in cereals.
- Results indicated that test strips had a higher false negative rate (25.7%-37.4%) than false positive rate (0%-31.0%), and ELISA kits showed varying repeatability depending on the concentration of mycotoxins tested.
- The study highlights the need for better antibody quality and more extensive performance testing to improve on-site mycotoxin detection methods in the future.
Article Abstract
Immunoassay-based techniques are important on-site screening tools for the detection of mycotoxins in cereals. This study aims to evaluate the trueness, precision, repeatability and cross-reactivity of commercially available test strips, ELISA kits and UHPLC-MS/MS on analyzing zearalenone, ochratoxin A, deoxynivalenol, T-2 toxin and fumonisin B. The results showed that false negative rate (25.7 %-37.4 %) of all tested mycotoxins by test strips was higher than the false positive rate (0 %-31.0 %). The repeatability of ELISA kits at the declared LOD dispersed from -85.7 % to +98.4 %. ELISA kits were more accurate at 50 % of the maximum residue limit (MRL) of mycotoxins than 150 % and 200 %. All the tested deoxynivalenol/zearalenone derivatives had cross-reactivity with different level, and sample matrix could reinforce this overestimation of target mycotoxin. This study emphasized that higher-quality antibody screening and more analytical performance investigations are need to address for on-site detection of mycotoxins in the future.
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| http://dx.doi.org/10.1016/j.foodchem.2022.135056 | DOI Listing |
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