Purpose: The purpose of this study was to assess the potential of new lipoglycopeptides as novel topical therapies for improved treatment of recalcitrant ocular infections. We evaluated the in vitro antimicrobial activity of oritavancin, dalbavancin, and telavancin compared with vancomycin (VAN) against a large collection of ocular staphylococcal isolates and their cytotoxicity on human corneal epithelial cells (HCECs).
Methods: Antimicrobial susceptibility testing was performed by broth microdilution against 223 Staphylococcus spp. clinical isolates. Time-kill kinetics were determined for methicillin-resistant strains of Staphylococcus aureus (MRSA) (n = 2) and Staphylococcus epidermidis (MRSE) (n = 1). In vitro cytotoxicity assays were performed with AlamarBlue and live/dead staining on HCECs.
Results: All new lipoglycopeptides showed strong in vitro potency against ocular staphylococci, including multidrug-resistant MRSA strains, with dalbavancin showing a slightly higher potency overall [minimum inhibitory concentration (MIC) 90 0.06 μg/mL] compared with telavancin and oritavancin (MIC 90 0.12 μg/mL), whereas VAN had the lowest potency (MIC 90 2 μg/mL). Oritavancin exerted rapid bactericidal activity within 1 h for MRSA and 2 h for MRSE. All other drugs were bactericidal within 24 h. At a concentration commonly used for topical preparations (25 mg/mL), cytotoxicity was observed for VAN after 5 min of incubation, whereas reduction in HCEC viability was not seen for telavancin and was less affected by oritavancin and dalbavancin. Cytotoxicity at 25 mg/mL was seen for all drugs at 30 and 60 min but was significantly reduced or undetected for lower concentrations.
Conclusions: Our study demonstrates that new lipoglycopeptides have substantially better in vitro antimicrobial activity against ocular staphylococcal isolates compared with VAN, with a similar or improved toxicity profile on HCECs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10060036 | PMC |
| http://dx.doi.org/10.1097/ICO.0000000000003197 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Improving Antimicrobial Utilization and Infection Control in Ophthalmology: An Information-Assisted Transparent Supervision and Multidisciplinary Team Model.
Infect Drug Resist
November 2024
Department of Nosocomial Infection Management, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, People's Republic of China.
Background: Using antimicrobials wisely is crucial for effective treatment and reducing antimicrobial resistance (AMR). As ocular infections can lead to serious consequences and ophthalmic surgery has a great impact on patients, the application of antimicrobials in ophthalmology needs to be managed in a standardized manner.
Methods: A multidisciplinary team (MDT) on antimicrobial stewardship was set up by adopting comprehensive management measures and a continuous improvement model with all-staff training and empowerment, information-assisted medical prescription control, and transparent supervision.
Evaluation of the Microbiology of Removed Punctal Plugs and Intracanalicular Devices.
Eye Contact Lens
November 2024
Department of Ophthalmology (J.J.M., N.E., W.C.F., A.M.F.), University of North Carolina, Chapel Hill, NC; Department of Ophthalmology (J.J.M.), University of Auckland, Auckland, New Zealand; Tricore Reference Laboratories (K.C.), Albuquerque, NM; Brooke Army Medical Center (E.A.), San Antonio, TX; Medical College of Wisconsin Department of Ophthalmology (N.E.), Milwaukee, WI; Campbell University School of Medicine (W.C.F.), Lillington, NC; Department of Pathology-Laboratory Medicine (P.G.), University of North Carolina School of Medicine, Chapel Hill, NC; and Department of Ophthalmology (A.M.F.), Duke University, Durham, NC.
Article Synopsis
- The study aimed to analyze the types of microorganisms that colonize punctal plugs and intracanalicular stents used for tear insufficiency and eye surgeries.
- The researchers collected and cultured 181 devices from participants and found that over half (54%) were culture positive, revealing a variety of microorganisms, mainly skin and upper respiratory flora.
- No clinical infections were reported, but the findings suggest that these ocular devices can harbor potentially harmful bacteria, indicating a need for monitoring.
Decade-long analysis of postoperative endophthalmitis in Sweden: Insights from insurance and national quality registers.
Heliyon
October 2024
Division of Eye and Vision. Department of Clinical Neuroscience, Karolinska Institutet, Stockholm, Sweden.
Purpose: To analyse clinical characteristics and risk factors for post-operative endophthalmitis (POE) in a Swedish patient insurance database and compare prevalence of POE with two national quality registers.
Methods: We analysed 218 medical records of patients who claimed compensation for POE following ocular surgery from 2010 to 2020. The data were cross-referenced with national quality registers.
Evaluation of microbial-induced calcite precipitation performance for soil surface improvement and toxicity assessment of the biostabilizer.
Heliyon
August 2024
Department of Energy and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
Microbial-induced calcite precipitation (MICP) is an environmentally friendly process that can be used to enhance soil surface stability against wind erosion. In this study, the performance of the MICP process on soil surface improvement was investigated using IR-103 bacteria. The biostabilizer, containing suspension and a cementation solution consisting of 0.
View Article and Find Full Text PDFInvestigation of antimicrobial resistance and antimicrobial resistance genes in and coagulase-negative staphylococci isolated from rabbit.
Vet World
June 2024
Department of Pre-clinic and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, 73710 Nakorn Pathom, Thailand.
Background And Aim: Staphylococci, which inhabit skin and mucous membranes in humans and animals, are opportunistic pathogens. Coagulase-positive and coagulase-negative staphylococci (CoNS) are the two main groups. Clinical abscesses in rabbits often harbor and CoNS.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!