The gut microbiome is essential for human health. Mouse microbiota models, including gnotobiotic mice, are the most prominent tools to elucidate the functions of gut bacteria. Here, we propose a targeted-bacterium-depleted (TBD) model using lytic bacteriophage to selectively deplete gut bacterium of healthy or otherwise defined mice. These phage-treated animals should have a near-complete spectrum of gut bacteria except for the depleted bacterium. To prove the concept, we employed -specific phage T7 to repress in the healthy mice. Our results showed that the depleted mice exhibited bravery-like behaviors, correlated to the presence of rather than the equilibrium among gut bacteria. Thus, we demonstrate that the TBD model is a powerful tool to elucidate the function of a specific bacterial species within a near-intact gut microbiota environment and complements gnotobiotic mice models.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9701607PMC
http://dx.doi.org/10.1016/j.crmeth.2022.100324DOI Listing

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