Robust solvatochromic carbon quantum dots for selective detection of water and Sn and specific lipid imaging.

Developing carbon quantum dots (CQDs) with the solvatochromic effect and exploring multifunctional applications remains challenging. Herein, robust solvatochromic carbon quantum dots (RS-CQDs) with emission shift up to ∼62 nm from yellow to red was fabricated by the hydrothermal method. The RS-CQDs was used to detect water and Sn in the linear ranges and limits of detection of 2.0-97.6% and 0.14% and 6.24-53.18 μM and 66.3 nM, respectively, and was further applied to determine Sn in practical water samples with satisfactory results. In addition, RS-CQDs exhibited bright red emission in oil media with a 9.7-fold increase in fluorescence relative to aqueous media, making them a wash-free probe for specifically staining lipids. Compared to the commercial lipid marker BODIPY 493/503, the RS-CQDs-based probe has significant advantages, such as longer emission, larger Stokes shift, and better photostability, ensuring that RS-CQDs-based marker can implement real-time and wash-free monitoring and imaging of lipids in living cells, liver tissues, zebrafish embryos, and zebrafish larvae. This study provides a novel research direction for the development of metal-doped CQDs by demonstrating RS-CQDs as the viability of fluorescence probes for water and Sn detection and the efficiency of RS-CQDs as a fluorescent marker for lipid imaging.