A plain, blank canvas does not look very beautiful; to make it aesthetically appealing requires adding structure and complexity. But how much structure is best? In other words, what is the relationship between beauty and complexity? It has long been hypothesized that complexity and beauty meet at a "sweet spot," such that the most beautiful images are neither too simple nor too complex. Here, we take a novel experimental approach to this question, using an information-theoretic approach to object representation based on an internal "skeletal" structure. We algorithmically generated a library of two-dimensional polygons and manipulated their complexity by gradually smoothing out their features-essentially decreasing the amount of information in the objects. We then stylized these shapes as "paintings" by rendering them with artistic strokes, and "mounted" them on framed canvases hung in a virtual room. Participants were shown pairs of these mounted shapes (which possessed similar structures but varied in skeletal complexity) and chose which shape looked best by previewing each painting on the canvas. Experiment 1 revealed a "Goldilocks" effect: participants preferred paintings that were neither too simple nor too complex, such that moderately complex shapes were chosen as the most attractive paintings. Experiment 2 isolated the role of complexity per se: when the same shapes were scrambled (such that their structural complexity was undermined, while other visual features were preserved), the Goldilocks effect was dramatically diminished. These findings suggest a quadratic relationship between aesthetics and complexity in ways that go beyond previous measures of each and demonstrate the utility of information-theoretic approaches for exploring high-level aspects of visual experience.
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STAR Protoc
January 2025
Department of Experimental Vascular Medicine, Amsterdam UMC, location AMC, Meibergdreef 9, Amsterdam, the Netherlands; Amsterdam Cardiovascular Sciences, Atherosclerosis & Ischemic Syndromes, Amsterdam, the Netherlands; Laboratory of Angiogenesis and Vascular Metabolism, VIB-KU Leuven Center for Cancer Biology, VIB, 3000 Leuven, Belgium; Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven and Leuven Cancer Institute (LKI), 3000 Leuven, Belgium. Electronic address:
The endothelium is the gatekeeper of vessel health, and its dysfunction is pivotal in driving atherogenesis. Here, we present a protocol to replicate endothelial-macrophage crosstalk during atherogenesis, called the "atherogenesis-on-chip" model, based on the Emulate dual-channel perfusion system. We describe a model for studying endothelial-macrophage interactions during atherogenesis in human aortic endothelial cells and human macrophages using qPCR and secretome analysis, fluorescence microscopy, and flow cytometry.
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January 2025
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China. Electronic address:
The plastid-encoded RNA polymerase (PEP) plays an essential role in the transcription of the chloroplast genome. Here, we present a strategy to purify the transcriptionally active protein complex from transplastomic tobacco (Nicotiana tabacum) lines in which one of the PEP core subunits is fused to an epitope tag. We describe experimental procedures for designing transformation constructs for PEP purification, selection, and analysis of transplastomic tobacco plants.
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January 2025
Laboratory of Developmental Neurobiology, International Institute of Molecular Mechanisms and Machines, 02-247 Warsaw, Poland; Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland. Electronic address:
Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. Here, we present an approach for rapamycin microinjection into the habenula of larval zebrafish to achieve localized inhibition of the mTorC1 pathway and explore the role of mTorC1 in habenula function. We describe steps for performing microinjections and maintaining zebrafish larvae before and after the procedure.
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January 2025
Smurfit Institute of Genetics, Trinity College Dublin, Dublin 2, Ireland. Electronic address:
Polycomb repressive complex 2 (PRC2), composed of the core subunits EED, SUZ12, and either EZH1 or EZH2, is critical for maintaining cellular identity in multicellular organisms. PRC2 deposits H3K27me3, which is thought to recruit the canonical form of PRC1 (cPRC1) to promote gene repression. Here, we show that EZH1-PRC2 and cPRC1 are the primary Polycomb complexes on target genes in non-dividing, quiescent cells.
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January 2025
School of Infection, Inflammation and Immunology, College of Medical and Dental Sciences, University of Birmingham, Birmingham, UK. Electronic address:
Interleukin (IL)-7 promotes T cell expansion during lymphopenia. We studied the metabolic basis in CD4 T cells, observing increased glucose usage for nucleotide synthesis and oxidation in the tricarboxylic acid (TCA) cycle. Unlike other TCA metabolites, glucose-derived citrate does not accumulate upon IL-7 exposure, indicating diversion into other processes.
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