Co-Existence of KPC-2, LAP-2, and CTX-M-65 in an ST1469 Multidrug-Resistant Strain in China.

Purpose: Beta-lactamase-producing is common in the clinic, but research associated with the co-existence of KPC-2, LAP-2, and CTX-M-65 in is still rare. In this study, the phenotypic and genetic characteristics of a multidrug-resistant strain SJ25 co-harboring , , and with rare ST1469 were investigated.

Methods And Results: Antimicrobial susceptibility testing revealed that strain SJ25 was resistant to various common antibiotics, except ciprofloxacin, fosfomycin, colistin, and tigecycline. Whole-genome analysis revealed that strain SJ25 carries a variety of antimicrobial resistance genes and virulence determinants. Plasmid analysis confirmed that the and genes were located on an ~136 kb transferrable IncFII/IncR plasmid and that was located on an untypeable plasmid.

Conclusion: Our findings emphasized the need for continuous surveillance of β-lactamase-bearing in the clinic to control potential dissemination and outbreak.