A biogas slurry composed of carbon, nitrogen, phosphorus, and antibiotics was generated. Investigations into the nutrient and tetracycline removal performance of four microalgae-based contaminant removal technologies, including Chlorella vulgaris, C. vulgaris co-cultured with endophytic bacteria, C. vulgaris co-cultured with Ganoderma lucidum, and C. vulgaris co-cultured with G. lucidum and endophytic bacteria, were conducted. The algal-bacterial-fungal consortium with 10 M strigolactone (GR24) yielded the maximum growth rate and average daily yield for algae at 0.325 ± 0.03 d and 0.192 ± 0.02 g L d, respectively. The highest nutrient/ tetracycline removal efficiencies were 83.28 ± 7.95 % for chemical oxygen demand (COD), 82.62 ± 7.97 % for total nitrogen (TN), 85.15 ± 8.26 % for total phosphorus (TP) and 83.92 ± 7.65 % for tetracycline. Adding an algal-bacterial-fungal consortium with an optimal synthetic analog GR24 concentration is seemingly an encouraging strategy for enhancing pollutant removal by algae, possibly overcoming the challenges of eutrophication and antibiotic pollution.
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