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Detection of MicroRNAs in Brain Slices Using In Situ Hybridization. | LitMetric

Detection of MicroRNAs in Brain Slices Using In Situ Hybridization.

Methods Mol Biol

Discipline of Physiology, School of Medicine, Trinity College Dublin, The University of Dublin, Dublin 2, Ireland.

Published: November 2022

AI Article Synopsis

Article Abstract

MicroRNAs are key posttranscriptional regulators of protein levels in cells. The brain is particularly enriched in microRNAs, and important roles have been demonstrated for these noncoding RNAs in various neurological disorders. To this end, visualization of microRNAs in specific cell types and subcellular compartments within tissue sections provides researchers with essential insights that support understanding of the cell and molecular mechanisms of microRNAs in brain diseases. In this chapter we describe an in situ hybridization protocol for the detection of microRNAs in mouse brain sections, which provides cellular resolution of the expression of microRNAs in the brain.

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Source
http://dx.doi.org/10.1007/978-1-0716-2823-2_6DOI Listing

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