AI Article Synopsis
- Exosomes are small vesicles released by cells that participate in various biological processes, especially in cancer progression and communication between cells.
- They carry molecules like exo-miRNAs, which influence tumor growth and the surrounding environment, making them valuable for research as potential biomarkers.
- The text includes a comprehensive protocol for isolating exosomes from different sources, verifying their identity, and analyzing the miRNAs they contain to ensure sample quality.
Article Abstract
Exosomes are extracellular vesicles secreted by cells with a key role in a wide range of biological processes including cancer. These vesicles are involved in intercellular communication and deliver diverse cargo molecules, including miRNAs (exo-miRNAs), to recipient cells affecting their physiology. Exo-miRNAs have a role in promoting tumor, progression, metastatization, and remodeling of tumor microenvironment, therefore making them interesting biomarkers to study.Here we provide a detailed technical protocol for exosome isolation (which can be applied to cell culture as well as physiological fluids), validation of their vesicular identity, miRNA extraction, and quantitative and qualitative analysis to evaluate the sample purity and concentration.
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| http://dx.doi.org/10.1007/978-1-0716-2823-2_5 | DOI Listing |
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