Analysis of Genes Related to Blackcurrant Reversion Virus Resistance in the Comparative Transcriptome of cv. Aldoniai.

Plants (Basel)

Lithuanian Research Centre for Agriculture and Forestry, Institute of Horticulture, Kaunas Str. 30, 54333 Babtai, Lithuania.

Published: November 2022

Blackcurrant reversion virus (BRV) is the most destructive mite-transmitted pathogen in blackcurrants. The understanding of the resistance to BRV is limited, hindering and delaying the selection process. To identify the resistance () gene for BRV resistance, a gene expression analysis based on de novo blackcurrant cv. Aldoniai comparative transcriptome analysis (mock- and BRV-inoculated samples at 2 and 4 days post-inoculation (dpi)) was performed. In this study, 111 annotated clusters associated with pathogenesis according to conservative gene domains were identified. In virus-infected samples, only Cluster-12591.33361 showed significant expression at 4 dpi. The expression profiles of this cluster were significantly associated with the presence of BRV particles in plant tissues, making it a putative gene in the dominant resistance strategy in the BRV- interaction. The newly identified gene _ belongs to the CC-NBS-LRR class and has 63.9% identity with in spp. This study provides new insights on dominant putative genes related to resistance to BRV in , which could aid targeted research and genetic improvement in breeding programs of blackcurrants.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692259PMC
http://dx.doi.org/10.3390/plants11223137DOI Listing

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