AI Article Synopsis
- Limitations in detecting cocirculating flaviviruses like Dengue and Zika led to the development of a new method combining aptameric capture with RT-PCR (APTA-RT-PCR).
- The aptamers were created using SELEX and next-gen sequencing, resulting in the design of a specific molecular beacon (APTAZC10-MB) that targets these viruses.
- APTA-RT-PCR successfully detected Dengue RNA in 43% and Zika RNA in 8% of tested serum samples, suggesting its potential for enhancing virus detection and improving infectious disease surveillance.
Article Abstract
Limitations in the detection of cocirculating flaviviruses such as Dengue and Zika lead us to propose the use of aptameric capture of the viral RNA in combination with RT-PCR (APTA-RT-PCR). Aptamers were obtained via SELEX and next-generation sequencing, followed by colorimetric and fluorescent characterizations. An APTA-RT-PCR assay was developed, optimized, and tested against the viral RNAs in 108 serum samples. After selection, sequence APTAZC10 was designed as a bifunctional molecular beacon (APTAZC10-MB), exhibiting affinity for the viral targets. APTA-RT-PCR was able to detect Dengue and Zika RNA in 43% and 8% of samples, respectively. Our results indicate that APTAZC10-MB and APTA-RT-PCR will be useful to improve the detection of Dengue and Zika viruses in a fast molecular assay for the improvement of infectious disease surveillance.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9693377 | PMC |
| http://dx.doi.org/10.3390/ijms232213866 | DOI Listing |
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