Superoxide dismutases (SODs) belong to the group of metalloenzymes that remove superoxide anion radicals and they have been identified in three domains of life: , and . SODs in sp. PCC 6803, CCALA 979, and sp. ZHR1A were investigated. We hypothesized that iron (FeSOD) and/or manganese (MnSOD) dominate as active forms in these cyanobacteria. Activity staining and three different spectroscopic methods of SOD activity bands excised from the gels were used to identify a suitable metal in the separated samples. FeSODs or enzymes belonging to the Fe-MnSOD superfamily were detected. The spectroscopic analyses showed that only Fe is present in the SOD activity bands. We found FeSOD in sp. PCC 6803 while two forms in and sp. ZHR1A: FeSOD1 and FeSOD2 were present. However, no active Cu/ZnSODs were identified in and sp. ZHR1A. We have shown that selected spectroscopic techniques can be complementary to the commonly used method of staining for SOD activity in a gel. Furthermore, the occurrence of active SODs in the cyanobacteria studied is also discussed in the context of SOD evolution in oxyphotrophs.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9692673 | PMC |
| http://dx.doi.org/10.3390/ijms232213819 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Enzymatic Antioxidants and Their Differential Response to Cryopreservation in Japanese Quail Semen.
Reprod Domest Anim
January 2025
Department of Zoology, Wildlife and Fisheries, Pir Mehr Ali Shah Arid Agriculture University of Rawalpindi, Rawalpindi, Pakistan.
A triad of enzymatic antioxidants viz., catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) constitutes a first line of defence against any redox imbalances in the semen. Cryopreservation enabling long term storage of semen also prompts generation of surplus reactive oxygen species (ROS) in the cells with waned antioxidants, hampering the full exploitation of this process.
View Article and Find Full Text PDFLaminated Layer Extract from Echinococcus Granulosus cyst Attenuates Ocular Damages and Inflammatory Responses in an Experimental Autoimmune Uveitis Model.
Acta Parasitol
January 2025
Cytokines and NO Synthases Team, LBCM, FSB, USTHB, BP 32 El Alia, Bab Ezzouar, Algiers, 16111, Algeria.
Purpose: Since extract of the laminated layer (LL) from E. granulosus showed immuno-modulatory effects in vitro and in vivo, we sought to determine its effect on the onset, development, and evolution of experimental auto-immune uveitis (EAU). The latter is a model of some human diseases with ocular inflammation that can cause blindness.
View Article and Find Full Text PDFBacopa monnieri Extract Diminish Hypoxia-Induced Anxiety by Regulating HIF-1α Signaling and Enhancing the Antioxidant Defense System in Hippocampus.
Neuromolecular Med
January 2025
Biochemistry and Molecular Biology Laboratory, Department of Zoology, Institute of Science, Banaras Hindu University, Varanasi, 221 005, India.
Hypoxia is a significant stressor, and stabilized hypoxia-inducible factor-1α (HIF-1α) regulates the expression of numerous genes, leading to various biochemical, molecular, physiological and genomic changes. The body's oxygen-sensing system activates gene expression to protect brain tissues from hypoxia. Gamma-aminobutyric acid, an inhibitory neurotransmitter, regulates brain excitability during hypoxia through the activation of HIF-1 α.
View Article and Find Full Text PDFEffects of Polystyrene Microplastic Exposure on Liver Cell Damage, Oxidative Stress, and Gene Expression in Juvenile Crucian Carp ().
Toxics
January 2025
Guangxi Key Laboratory of Theory and Technology for Environmental Pollution Control, Guilin University of Technology, Guilin 541006, China.
A considerable quantity of microplastic debris exists in the environment and the toxicity of these materials has a notable impact on aquatic ecosystems. In this paper, 50-500 µm polystyrene microplastics (exposure concentrations were 200 µg/L, 800 µg/L, and 3200 µg/L concentrations) were selected to study the effects of polystyrene microplastics (PS-MPs) on cell morphology, detoxification enzyme activity, and mRNA expression in the liver tissues of crucian carp juveniles. The results demonstrated that: (1) Different concentrations of PS-MPs cause varying degrees of pathological and oxidative damage to liver tissue cells of crucian carp.
View Article and Find Full Text PDFNeurotoxicological Effects of Some Mycotoxins on Humans Health and Methods of Neuroprotection.
Toxins (Basel)
January 2025
Department of Preclinical Sciences and Infectious Diseases, Faculty of Veterinary Medicine and Animal Sciences, Poznan University of Life Sciences, Wolynska St. 35, 60-637 Poznan, Poland.
Food contamination with mycotoxin-producing fungi increases the risk of many diseases, including neurological diseases closely related to the neurotoxicity of these toxins. Based on the latest literature data, we presented the association of common mycotoxins with neurological diseases. Articles from 2001 to 2024 were analyzed.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!