AI Article Synopsis
- Cytokeratin and desmin are proteins linked to Sertoli cell development and testicular germ cell cancer (TGCC), with this study focusing on their expression in normal and cancerous testicular tissue.
- The study found that desmin is present in Sertoli cells during fetal development but decreases significantly by the second trimester, and it remains in most adult Sertoli cells, even in TGCC cases.
- Cytokeratin is found in fetal Sertoli cells but absent in adult Sertoli cells, indicating it may signal issues with Sertoli cell maturity, especially in cases of TGCC.
Article Abstract
Cytokeratin and desmin expression have been associated with Sertoli cell maturity and the development of testicular germ cell cancer (TGCC). Thus, the present study aimed to characterize the expression of these intermediate filaments in normal testis development and TGCC. Cytokeratin and desmin were determined by immunohistochemistry and immunofluorescence in human fetal, and adult testis and tissue from patients with pre-invasive germ cell neoplasia in-situ (GCNIS) or invasive TGCC. Desmin was expressed in Sertoli cells of the human fetal testis, and the proportion of desmin expressing Sertoli cells was significantly reduced in the second trimester, compared with the first trimester (31.14% vs. 6.74%, 0.0016). Additionally, Desmin was expressed in the majority of Sertoli cells in the adult testis and TGCC samples. Cytokeratin was detected in Sertoli cells of human fetal testis but was not expressed in Sertoli cells of human adult testis. In patients with TGCC, cytokeratin was not expressed in Sertoli cells in tubules with active spermatogenesis but was detected in Sertoli cells in tubules containing GCNIS cells in patients with both pre-invasive and invasive TGCC. In conclusion, desmin was not associated with Sertoli cell maturation or progression to TGCC. However, cytokeratin appeared to be an indicator of impaired Sertoli cell maturation.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9688874 | PMC |
| http://dx.doi.org/10.3390/cancers14225479 | DOI Listing |
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