Effect of Bacteriocin-Like Inhibitory Substance (BLIS) from DB1 on Cariogenic Biofilm Formation.

The aim of the study was to investigate the effect of bacteriocin-like inhibitory substance (BLIS) from DB1 on cariogenic biofilm. Crystal violet staining, fluorescence, and scanning electron microscopy analyses demonstrated that the BLIS from DB1 (DB1 BLIS) inhibited biofilm. When DB1 BLIS was co-incubated with , biofilm formation by was significantly reduced (p<0.05). DB1 BLIS also destroyed the preformed biofilm of . In addition, DB1 BLIS decreased the viability of biofilm cells during the development of biofilm formation and in the preformed biofilm. DB1 BLIS significantly decreased the growth of planktonic cells. Furthermore, biofilm on the surface of saliva-coated hydroxyapatite discs was reduced by DB1 BLIS. Taken together, DB1 BLIS might be useful as a preventive and therapeutic agent against dental caries caused by .