Protein phosphorylation is one of the most widely studied posttranslational modifications, and its role in signal transduction has gained particular attention. The relatively low abundance of the phosphorylated form of proteins makes identification by mass spectrometry challenging in the absence of selective enrichment. Titanium oxide-based enrichment of phosphopeptides in the presence of acidic modifiers is highly selective and makes it technically possible to detect thousands of phosphopeptides in a single sample by liquid chromatography-mass spectrometry. In this chapter, we describe a detailed protocol for the selective enrichment of microsomal and cytosolic phosphopeptides from Arabidopsis seedlings. The resulting phosphopeptide fractions enable routine identification of several thousands of phosphopeptide spectra per sample by mass spectrometry.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-0716-2784-6_18 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Experimental and explainable machine learning approach on thermal conductivity and viscosity of water based graphene oxide based mono and hybrid nanofluids.
Sci Rep
December 2024
Department of Mechanical Engineering, Delhi Skill and Entrepreneurship University, Delhi, 110089, India.
This study explores the thermal conductivity and viscosity of water-based nanofluids containing silicon dioxide, graphene oxide, titanium dioxide, and their hybrids across various concentrations (0 to 1 vol%) and temperatures (30 to 60 °C). The nanofluids, characterized using multiple methods, exhibited increased viscosity and thermal conductivity compared to water, with hybrid nanofluids showing superior performance. Graphene oxide nanofluids displayed the highest thermal conductivity and viscosity ratios, with increases of 52% and 177% at 60 °C and 30 °C, respectively, for a concentration of 1 vol% compared to base fluid.
View Article and Find Full Text PDFGuest modulating the photoactivity of a titanium-oxide cage.
Chem Sci
December 2024
Key Lab for Colloid and Interface Science of Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University Jinan 250100 China
Two host-guest Ti-oxide clusters, Ti(NH) and TiCs, were synthesized and thoroughly characterized. They possess a rarely seen biloculate structure that encapsulates two NH and Cs guests, respectively. Interestingly, alkali metal cations can exchange places with NH .
View Article and Find Full Text PDFGraphene Oxide-Doped Indium Oxide Buffer Film Sandwiched between Titanium Oxide Layers for the Development of Photosensitive Resistive Memory Devices.
ACS Appl Mater Interfaces
November 2024
College of Electrical and Computer Engineering, Chungbuk National University, Cheongju 28644, Korea.
Over the past decade, metal oxide semiconductors have attracted considerable attention because of their transparency, high intrinsic charge carrier mobility, and charge carrier density. Metal oxide semiconductors also provide a promising route to develop resistive memory devices because of the tunability of their conductivity via the removal of oxygen ions, forming oxygen vacancies that can act as electron donors. Here, this paper reports the fabrication of a resistive random-access memory (ReRAM) device with TiO and TiO layers and introduces a solution-processed InO-graphene oxide (GO) buffer layer from a water-based precursor solution to tune the switching characteristics.
View Article and Find Full Text PDFVitalizing Perovskite Oxide-Based Acetone Sensors with Metal-Organic Framework-Derived Heterogeneous Oxide Catalysts.
ACS Sens
December 2024
Department of Materials Science and Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Article Synopsis
- Perovskite oxides are effective for gas sensors due to their stability, but they have issues like low surface area and reactivity that impair their performance.
- By incorporating metal-organic framework (MOF)-derived α-FeO nanoparticles with platinum catalysts into a p-type LaCaFePtO perovskite, the researchers created a composite with enhanced surface activity.
- This new composite showed exceptional sensitivity to acetone gas at high temperatures, highlighting the importance of interfacial engineering in improving the performance of perovskite-based gas sensors.
Interface engineering of Platinum-Copper alloy/titanium dioxide for enhanced photocatalytic carbon dioxide reduction.
Environ Res
December 2024
State Key Laboratory of Pollution Control and Resource Reuse, Jiangsu Key Laboratory of Vehicle Emissions Control, School of Environment, Nanjing University, Nanjing, 210023, PR China. Electronic address:
To develop an efficient photocatalytic carbon dioxide (CO) reduction aimed at mitigating CO emissions and greenhouse effects, we propose a straightforward strategy involving hydrogen reduction treatment of PtCu/Ti to create the PtCu/Ti-H catalyst with a distinctive interface structure. Compared with the fresh PtCu/Ti catalyst and the benchmark anatase TiO, the CH production of the PtCu/Ti-H catalyst increased by 2 times and 81.6 times, respectively.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!