AI Article Synopsis
- Autophagy is essential for degrading and recycling proteins and organelles in eukaryotic cells, playing a critical role in plant stress responses, immunity, and cellular maintenance.
- During autophagy, structures called autophagosomes form to transport materials to the vacuole for degradation, with their quantity serving as an indicator of autophagy activity.
- Two common methods to detect autophagosomes include using the fluorescent dye monodansylcadaverine for staining and expressing a GFP fusion with the ATG8 protein that is found in the autophagosomal membrane.
Article Abstract
Autophagy is a key process for degradation and recycling of proteins or organelles in eukaryotes. Autophagy in plants has been shown to function in stress responses, pathogen immunity, and senescence, while a basal level of autophagy plays a housekeeping role in cells. Upon activation of autophagy, vesicles termed autophagosomes are formed to deliver proteins or organelles to the vacuole for degradation. The number of autophagosomes can thus be used to indicate the level of autophagy. Here we describe two common methods used for detection of autophagosomes, staining of autophagosomes with the fluorescent dye monodansylcadaverine and expression of a fusion between GFP and the autophagosomal membrane protein ATG8.
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| http://dx.doi.org/10.1007/978-1-0716-2784-6_11 | DOI Listing |
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