AI Article Synopsis
- The study investigates the impact of whole-body gamma irradiation on rat lungs, focusing on histopathological changes, enzymatic alterations, and DNA damage, while assessing the potential protective role of pycnogenol.
- A total of 100 adult male rats were divided into ten groups to examine various doses of pycnogenol before and after exposure to radiation, specifically measuring catalase (CAT) and superoxide dismutase (SOD) activities, along with malondialdehyde (MDA) levels in lung tissues.
- Results showed that while gamma irradiation reduced CAT and SOD activities and increased MDA levels, the group treated with pycnogenol exhibited improved enzymatic activity and lower DNA damage, indicating its significant protective effect
Article Abstract
Background And Objective: The study aims to evaluate the histopathological changes, enzymatic alterations, and DNA damage in rat lungs induced by whole-body gamma irradiation as well as evaluation of the protective effect of pycnogenol.
Materials And Methods: A hundred adult male rats were equally divided into ten groups including control, four antioxidants, γ-irradiation, four antioxidant + γ-irradiations. This study began the day before radiation treatment and continued for 3 days. The pycnogenol was dissolved 5% dimethyl sulfoxide and then administered orally through a gastric tube at a dose of 37.5 mg/kg, 75 mg/kg, 150 mg/kg, and 300 mg/kg in 24, 48, and 72 h before irradiation. Irradiation was applied with a whole-body irradiation dose of 900 cGy in one fraction. DNA damage, histopathological changes, catalase (CAT), and superoxide dismutase (SOD) activities and malondialdehyde (MDA) levels in lung tissue of rats were evaluated 3 days after irradiation.
Results: CAT and SOD activities were found to be significantly lower in the irradiation group than control (P < 0.001). CAT and SOD activities were higher in the antioxidant + γ-irradiation group than both irradiation and control groups. MDA levels were significantly higher in the irradiation group compared to control (P < 0.001), whereas MDA levels decreased in the antioxidant + γ-irradiation group compared to the irradiation group. The antioxidant groups were significantly increased comet parameters depend on pycnogenol doses compared to control. The antioxidant + γ-irradiation was decreased comet parameter compared to γ-irradiation. As a result of the histopathologically, the antioxidant groups were different than the control group that in the areas of alveolar sacs and connective tissue areas were seen hemorrhage areas similar to the irradiation group.
Conclusion: We demonstrate that 300 mg/kg of pycnogenol might provide significant protection against deleterious effects from whole-body ionizing radiation on the lung tissue. P+ γ-ray group was significantly reduced radiation-induced lung injury and was possible to observe significantly preservation.
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| http://dx.doi.org/10.4103/jcrt.JCRT_1062_20 | DOI Listing |
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