Severity: Warning
Message: file_get_contents(https://...@pubfacts.com&api_key=b8daa3ad693db53b1410957c26c9a51b4908&a=1): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests
Filename: helpers/my_audit_helper.php
Line Number: 176
Backtrace:
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 176
Function: file_get_contents
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 250
Function: simplexml_load_file_from_url
File: /var/www/html/application/helpers/my_audit_helper.php
Line: 3122
Function: getPubMedXML
File: /var/www/html/application/controllers/Detail.php
Line: 575
Function: pubMedSearch_Global
File: /var/www/html/application/controllers/Detail.php
Line: 489
Function: pubMedGetRelatedKeyword
File: /var/www/html/index.php
Line: 316
Function: require_once
Objectives: Rheumatoid arthritis is a common autoimmune disease, and microRNAs (miRNAs) are involved in its pathogenesis. This study aims to examine the differentially expressed miRNAs in collagen-induced arthritis (CIA) rats, to analyze the biological functions and the related pathways of the miRNA target genes.
Methods: The total RNA in the synovium of experimental animals was extracted. The miRNA gene profile was obtained by miRNA microarray. Then the differentially expressed miRNAs were screened and the relevant target mRNAs were predicted. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed on the significantly differentially expressed miRNAs.
Results: There were 69 differentially expressed miRNAs including rno-miR-6215 and rno-miR-709 in CIA rats, of which 22 (31.9%) were up-regulated and 47 (68.1%) were down-regulated. GO and KEGG enrichment analysis showed that the up-regulated miRNA target genes were mainly enriched in cellular metabolism, and they were involved in MAPK and Wnt signaling pathways. The down-regulated miRNA target genes were mainly enriched in nervous system development, and they were involved in axon guidance signaling pathway.
Conclusions: There are differentially expressed miRNAs in the CIA rat model, which may be involved in metabolism biological functions and signal pathways such as MAPK and Wnt.
Download full-text PDF |
Source |
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10930329 | PMC |
http://dx.doi.org/10.11817/j.issn.1672-7347.2022.210789 | DOI Listing |
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