A species of Hook. f. et Thoms., 1861, which belongs to the Cardamine family (Brassicaceae), is an endemic species of the Wuling Mountains in Hunan and Hubei Provinces of China. Since there are many morphologically related species of , the chloroplast (cp) genome characteristics of were analyzed in order to explore the phylogenetic relationship between it and the closely related species. A cp genome totaling 154,838 base pairs exhibited a typical quadripartite structure with a pair of IRs (inverted repeats; 26,493 base pairs) separated by a small single-copy region of 17,938 base pairs and a large single-copy region of 83,914 base pairs. A total of 130 genes were found in the cp genome, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. There were 36.21%, 33.96%, 29.09%, and 42.35% GC content in the entire cp genome, LSC region, SSC region, and IR region, respectively. According to phylogenetic analysis, is evolutionarily closer to .
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http://dx.doi.org/10.1080/23802359.2022.2141081 | DOI Listing |
BMC Genomics
January 2025
State Key Laboratory of Tree Genetics and Breeding, National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China.
Background: Populus tomentosa, known as Chinese white poplar, is indigenous and distributed across large areas of China, where it plays multiple important roles in forestry, agriculture, conservation, and urban horticulture. However, limited accessibility to the mitochondrial (mt) genome of P. tomentosa impedes phylogenetic and population genetic analyses and restricts functional gene research in Salicaceae family.
View Article and Find Full Text PDFActa Histochem
January 2025
School of Chemistry, University of Glasgow, Glasgow G12 8QQ, UK.
In recent years, a great interest has been focused on the prebiotic origin of nucleic acids and life on Earth. An attractive idea is that life was initially based on an autocatalytic and autoreplicative RNA (the RNA-world). RNA duplexes are right-handed helical chains with antiparallel orientation, but the rationale for these features is not yet known.
View Article and Find Full Text PDFAnal Chim Acta
January 2025
Key Laboratory of Biomaterials of Guangdong Higher Education Institutes, Department of Biomedical Engineering, Jinan University, Guangzhou, 510632, China. Electronic address:
Background: The multifunctional cytokine interleukin-6 (IL-6) plays a pivotal role in chronic and acute inflammatory responses, underscoring the importance of accurately determining IL-6 levels for early diagnosis, prevention, and treatment of inflammation.
Results: This study developed a versatile and innovative single-particle surface-enhanced Raman spectroscopy (SERS) sensing platform for the precise and sensitive quantification of IL-6 in complex samples using a novel one-pot synthesized, silver ions-doped three-dimensional porous gold microparticles (PGMs) with abundant hot spots for robust SERS enhancement. By rationally designing rich cytosine-Ag-cytosine base pairs between IL-6 aptamers and complementary chains on the PGMs, we harnessed the SERS-enhancing effect to achieve highly sensitive and specific IL-6 quantification within a wide range of 10 to 10 mg/mL and a limit of detection (LOD) of 0.
Mikrochim Acta
January 2025
School of Science, Xihua University, Chengdu, 610039, People's Republic of China.
A dual-mode detection platform utilizing colorimetric and Raman was developed based on the exponential amplification reaction (EXPAR) strategy and a "core-satellite" structure constructed by bimetallic nanozymes to detect chloramphenicol (CAP). Initially, DNA-gated metal-organic frameworks (MOFs) incorporating cascaded amplification were used to be nanocarriers for the colorimetric and Raman reporter molecules (3,3',5,5'-tetramethylbiphenyl; TMB). Subsequently, assembled DNA served as gatekeepers to create a stimulus-responsive DNA-gated MOF (TMB@DNA/MOF).
View Article and Find Full Text PDFArch Microbiol
January 2025
Department of Critical Care Medicine, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, 637000, PR China.
Vibrio parahaemolyticus propels itself through liquids using a polar flagellum and efficiently swarms across surfaces or viscous environments with the aid of lateral flagella. H-NS plays a negative role in the swarming motility of V. parahaemolyticus by directly repressing the transcription of the lateral flagellin gene lafA.
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