Microbial community succession during crude oil-degrading bacterial enrichment cultivation and construction of a degrading consortium.

Microbial community succession during the enrichment of crude-oil-degrading bacteria was analyzed using Illumina high-throughput sequencing to guide bacterial isolation and construction of a bacterial consortium. Community change occurred in 6 days; the most abundant phylum changed from Proteobacteria to Actinobacteria; the most abundant genera were and . Two crude oil-degrading strains, sp. OS62-1 and sp. OS33, and one weak-crude-oil-degrading strain, sp. P35, were isolated. A consortium comprising sp. OS62-1 and sp. P35 showed the highest crude-oil-degrading efficiency, reaching 85.72 ± 3.21% within 7 days, over a wide pH range (5-11) and salinity (0-80 g·L). Consumption of saturated hydrocarbons, aromatic hydrocarbons, and resins was greater by the consortium than by a single strain, as was degradation of short-chain-alkanes (C-C) according to gas-chromatography. The bacterial consortium provides technical support for bioremediation of crude oil pollution.