Knockdown of Stromal Interaction Molecule 1 () Suppresses Acute Myeloblastic Leukemia-M5 Cell Line Survival Through Inhibition of Reactive Oxygen Species Activities.

Objective: This study aimed to investigate the role of the stromal interaction molecule 1 () gene in the survival of the acute myeloblastic leukemia (AML)-M5 cell line (THP-1).

Materials And Methods: The effect was assessed via dicersubstrate siRNA-mediated knockdown. The effect of knockdown on the expression of and pathway-related genes and reactive oxygen species (ROS) generation-related genes was tested using real-time polymerase chain reaction. Cellular functions, including ROS generation, cell proliferation, and colony formation, were also evaluated following knockdown.

Results: The findings revealed that knockdown reduced intracellular ROS levels via downregulation of and . These findings were associated with the downregulation of , and was also downregulated, while was upregulated following knockdown. Furthermore, knockdown reduced THP-1 cell proliferation and colony formation.

Conclusion: This study has demonstrated the role of in promoting AML cell proliferation and survival through enhanced ROS generation and regulation of AKT/MAPK-related pathways. These findings may help establish as a potential therapeutic target for AML treatment.