Optimization of agarose-alginate hydrogel bead components for encapsulation and transportation of stem cells.

Unlabelled: Hydrogels have potential uses in various biological applications because of their unique characteristics. Fine-tuning of agarose-alginate (Ag-Al) hydrogel components improves the mechanical characteristics of the final construct for cell encapsulation and transportation. Formulation of suitable dissolving agents may enable the release of encapsulated cells for further applications in laboratory or clinical settings.

Objectives: This study aimed at optimizing the composition of Ag-Al hydrogel beads and their dissolving agents for potential use in the transportation of stem cells.

Methods: Various agarose, alginate, and CaCl concentrations were tested to construct hydrogel beads. The degradation rate and swelling ratio of each hydrogel sample were recorded. The optimized Ag-Al hydrogels were used for encapsulation of stem cells from human exfoliated deciduous teeth (SHED). Optimization of dissolving agents was performed and tested with the hydrogel-encapsulated cells. Data were statistically analyzed in SPSS.

Results: The selected concentration of Ag-Al hydrogels components was successfully demonstrated to encapsulate SHED, which remained viable until day 10. An average of 2 min was required for degradation of the hydrogel with encapsulated SHED by a dissolving agent consisting of 100 mM sodium citrate and 100 mM EDTA. The cell viability of SHED released after day 10 of encapsulation was 29.1%.

Conclusion: Alteration of Ag-Al components has considerable influence on the mechanical properties of the constructed hydrogel. The feasibility of performing the optimized cell encapsulation protocol, as well as the dissolving step, may provide a useful guide for the transportation of viable cells between countries, for medical research.