AI Article Synopsis
- Babesia is often misdiagnosed as malaria due to similarities in appearance, which can lead to improper treatment in areas where both diseases are present.
- Optical diffraction tomography (ODT) allows researchers to create detailed 3D images of these parasites at the single-cell level, helping to better understand their differences and impacts on host cells.
- The study found significant morphological and biochemical differences between P. falciparum and B. microti, indicating that P. falciparum has a more pronounced effect on red blood cells, suggesting that accurate differentiation could improve clinical diagnosis and treatment strategies.
Article Abstract
Background: Babesia is an intraerythrocytic parasite often misdiagnosed as a malaria parasite, leading to inappropriate treatment of the disease especially in co-endemic areas. In recent years, optical diffraction tomography (ODT) has shown great potential in the field of pathogen detection by quantification of three-dimensional (3D) imaging tomograms. The 3D imaging of biological cells is crucial to investigate and provide valuable information about the mechanisms behind the pathophysiology of cells and tissues.
Methods: The early ring stage of P. falciparum were obtained from stored stock of infected RBCs and of B. microti were obtained from infected patients during diagnosis. The ODT technique was applied to analyze and characterize detailed differences between P. falciparum and B. microti ring stage at the single cell level. Based on 3D quantitative information, accurate measurement was performed of morphological, biochemical, and biophysical parameters.
Results: Accurate measurements of morphological parameters indicated that the host cell surface area at the ring stage in B. microti was significantly smaller (140.2 ± 17.1 µm) than that in P. falciparum (159.0 ± 15.2 µm), and sphericities showed higher levels in B. microti-parasitized cells (0.66 ± 0.05) than in P. falciparum (0.60 ± 0.04). Based on biochemical parameters, host cell hemoglobin level was significantly higher and membrane fluctuations were respectively more active in P. falciparum-infected cells (30.25 ± 2.96 pg; 141.3 ± 24.68 nm) than in B. microti (27.28 ± 3.52 pg; 110.1 ± 38.83 nm). The result indicates that P. falciparum more actively altered host RBCs than B. microti.
Conclusion: Although P. falciparum and B. microti often show confusable characteristics under the microscope, and the actual three-dimensional properties are different. These differences could be used in differential clinical diagnosis of erythrocytes infected with B. microti and P. falciparum.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9673388 | PMC |
| http://dx.doi.org/10.1186/s13071-022-05569-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Novel, standardized sample collection from the brain-nose interface.
Methods
January 2025
Noselab GmbH, Widenmayerstr. 27, 80538 Munich, Germany.
Background: Diagnostics for neurodegenerative diseases lack non-invasive approaches suitable for early-stage biochemical screening and routine examination of neuropathology. Biomarkers of neurodegenerative diseases pass through the brain-nose interface (BNI) and accumulate in nasal secretion. Sample collection from the brain-nose interface presents a compelling prospect as basis for a non-invasive molecular diagnosis of neuropathologies.
View Article and Find Full Text PDFSensitive fluorescence turn-on sensing of hydroxyl radical and glucose based on the oxidative degradation of reductive organic cage.
Talanta
January 2025
College of Chemistry and Materials Science, Hunan Engineering Research Center for Monitoring and Treatment of Heavy Metals Pollution in the Upper Reaches of Xiangjiang River, Hengyang Normal University, Hengyang, 421001, China. Electronic address:
The accurate and sensitive quantification of hydroxyl radical (·OH) and glucose is necessary for disease diagnosis and health guidance, but still challenging owing to the low concentration of ·OH and poor water solubility of fluorescent probes. In addition, fluorescent probes may cause secondary pollution to the environment. Here an organic cage was reported as a sensitive fluorescent probe for ·OH and glucose in aqueous solution without serious secondary pollution.
View Article and Find Full Text PDFA root system architecture regulator modulates OsPIN2 polar localization in rice.
Nat Commun
January 2025
State Key Laboratory of Plant Environmental Resilience, College of Life Sciences, Zhejiang University, Hangzhou, 310058, China.
Ideal root system architecture (RSA) is important for efficient nutrient uptake and high yield in crops. We cloned and characterized a key RSA regulatory gene, GRAVITROPISM LOSS 1 (OsGLS1), in rice (Oryza sativa L.).
View Article and Find Full Text PDFDupuytren's Contracture: A Review of the Literature.
Cureus
December 2024
Department of Orthopedics, Liverpool University Hospitals National Health Service (NHS) Foundation Trust, Liverpool, GBR.
Dupuytren's contracture is a chronic condition that affects the palmar fascia, leading to progressive flexion of the fingers, particularly the ring and little fingers. This article provides an in-depth review of the current understanding of the condition and its management. Commonly seen in older men of Northern European descent, Dupuytren's can significantly impair hand function as contractures develop.
View Article and Find Full Text PDFFirst report of tobacco root rot caused by in Guangdong of China.
Plant Dis
December 2024
Xuchang, China;
Tobacco ( L.) is an economically important crop in China. In April 2024, field tobacco (cv.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!